概述

• 产品名称Anti-Cdk6抗体[EPR4515]
     

• 描述

  兔单克隆抗体[EPR4515] to Cdk6

• 经测试应用ICC/IF,WB,IHC-P,ICC,Flow Cyt

• 种属反应性

  与反应: Human    
   

• 免疫原

  Synthetic peptide corresponding to residues in C-terminus of Human Cdk6.

• 阳性对照    

• Jurkat cell lysate, K562 cell lysate, HeLa cell lysate and 293T cell lysate. Human tonsil tissue. HeLa cells. ICC/IF: Cdk6 wildtype and knockout HAP1 cells

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

   

  Alternative versions available:

  Anti-Cdk6 antibody (Alexa Fluor^® 488) [EPR4515] (ab198944)
  Anti-Cdk6 antibody (Alexa Fluor^® 647) [EPR4515] (ab198946)

  Anti-Cdk6 antibody (HRP) [EPR4515] (ab198945)

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液pH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPR4515

• 同种型IgG

• 研究领域

• Cancer

• Cell cycle

• Kinases/phosphatases

• Cdks

• Epigenetics and Nuclear Signaling

• Cell cycle

• Kinases/Phosphatases

• Cdks

• Cell Biology

• Cell Cycle

• Kinases/Phosphatases

• Cdks

Anti-Cdk6 antibody [EPR4515] 图像

• 

  Western blot - Anti-Cdk6 antibody [EPR4515] (ab124821)          

     
  
  Predicted band size : 37 kDa
     

  Lanes 1, 3 and 5: Wild-type HAP1 cell lysate (20 µg)
  Lanes 2, 4 and 6: CDK6 knockout HAP1 cell lysate (20 µg)
  Lanes 1 and 2: Green signal from target - ab124821 observed at 37 kDa
  Lanes 3 and 4: Red signal from loading control - ab8226 observed at 42 kDa
  Lanes 5 and 6: Merged (red and green) signal
  ab124821 was shown to specifically react with CDK6 when CDK6 knockout samples were used. Wild-type and CDK6 knockout samples were subjected to SDS-PAGE. ab124821 and ab8226 (loading control to beta actin) were diluted at 1/10 000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

   

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cdk6 antibody [EPR4515] (ab124821)          

     

  ab124821 staining Cdk6 in wild-type HAP1 cells (top panel) and Cdk6 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab124821 at 1/500 dilution and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

   

• 

  Flow Cytometry - Anti-Cdk6 antibody [EPR4515] (ab124821)          

     Overlay histogram showing HeLa cells stained with ab124821 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124821, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.  

• 

  Western blot - Anti-Cdk6 antibody [EPR4515] (ab124821)          

     All lanes : Anti-Cdk6 antibody [EPR4515] (ab124821) at 1/1000 dilution
  
  Lane 1 : Jurkat cell lysate
  Lane 2 : K562 cell lysate
  Lane 3 : HeLa cell lysate
  Lane 4 : 293T cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat-anti-rabbit HRP at 1/2000 dilution
  
  Predicted band size : 37 kDa
  Observed band size : 37 kDa
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk6 antibody [EPR4515] (ab124821)          

     ab124821 at 1/100 dilution, staining Cdk6 in Formalin fixed Paraffin-embedded Human tonsil tissue by Immunohistochemistry.  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Cdk6 antibody [EPR4515] (ab124821)          

     ICC/IF image of ab124821 at 1/100 dilution, staining Cdk6 in HeLa cells.