概述

• 产品名称Anti-Cdk7 (phospho T170)抗体[EPR6650(2)]
     

• 描述

  兔单克隆抗体[EPR6650(2)] to Cdk7 (phospho T170)

• 特异性This antibody only detects CDK7 phosphorylated at threonine 170.

• 经测试应用WB

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Cdk7 (phospho T170).
  Database link: P50613

• 阳性对照    

• WB: HeLa, A431, NIH/3T3 and C6 cell lysates and rat spleen tissue lysate.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 40% Glycerol, 0.05% BSA, 59% PBS

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR6650(2)

• 同种型IgG

• 研究领域

• Epigenetics and Nuclear Signaling

• Cell cycle

• Kinases/Phosphatases

• Cdks

• Cell Biology

• Cell Cycle

• Kinases/Phosphatases

• Cdks

Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] 图像

• 

  Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)          

     All lanes : Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified)
  
  Lane 1 : HeLa cell lysate (untreated)
  Lane 2 : HeLa cell lysate (treated with Lambda phosphatase)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
  
  Predicted band size : 39 kDa
  Observed band size : 39 kDa
     

  Blocking buffer and concentration: 5% NFDM/TBST.

  Diluting buffer and concentration: 5% NFDM /TBST.

   

• 

  Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)          

     Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified) + A431 cell lysate at 20 µg
  
  Secondary
  Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
  
  Predicted band size : 39 kDa
  Observed band size : 39 kDa
     

  Blocking buffer and concentration: 5% NFDM/TBST.

  Diluting buffer and concentration: 5% NFDM /TBST.

   

• 

  Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)          

     Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified) + NIH/3T3 cell lysate at 20 µg
  
  Secondary
  Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
  
  Predicted band size : 39 kDa
  Observed band size : 39 kDa
     

  Blocking buffer and concentration: 5% NFDM/TBST.

  Diluting buffer and concentration: 5% NFDM /TBST.

   

• 

  Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)          

     All lanes : Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (purified)
  
  Lane 1 : C6 cell lysate
  Lane 2 : Rat spleen tissue lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
  
  Predicted band size : 39 kDa
  Observed band size : 39 kDa
     

  Blocking buffer and concentration: 5% NFDM/TBST.

  Diluting buffer and concentration: 5% NFDM /TBST.

   

• 

  Western blot - Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976)          

     All lanes : Anti-Cdk7 (phospho T170) antibody [EPR6650(2)] (ab155976) at 1/1000 dilution (unpurified)
  
  Lane 1 : HeLa cell lysate (untreated)
  Lane 2 : HeLa cell lysate (treated with Lambda phosphatase)
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 39 kDa