详细说明
概述
产品名称Anti-GLCNE抗体[EPR15059] - C-terminal
描述
兔单克隆抗体[EPR15059] to GLCNE - C-terminal
经测试应用ICC/IF,IP,WB
种属反应性
与反应: Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human GLCNE aa 700 to the C-terminus. The exact sequence is proprietary.
Database link: Q9Y223阳性对照
Human fetal brain, fetal kidney, fetal liver, HeLa, A549 and K562 lysates; HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR15059
同种型IgG
研究领域
Developmental Biology
Organogenesis
Hematopoietic system development
Cardiovascular
Blood
Other
Anti-GLCNE antibody [EPR15059] - C-terminal 图像
Western blot - Anti-GLCNE [EPR15059] antibody - C-terminal (ab189927)
All lanes : Anti-GLCNE antibody [EPR15059] - C-terminal (ab189927) at 1/1000 dilution
Lane 1 : Human fetal liver
Lane 2 : Human fetal kidney
Lane 3 : Human fetal brain
Lysates/proteins at 20 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 79 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Western blot - Anti-GLCNE [EPR15059] antibody - C-terminal (ab189927)
All lanes : Anti-GLCNE antibody [EPR15059] - C-terminal (ab189927) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 79 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Anti-GLCNE [EPR15059] antibody - C-terminal (ab189927)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling GLCNE with ab189927 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with DAPI.
Immunoprecipitation - Anti-GLCNE [EPR15059] antibody - C-terminal (ab189927)
Western blot analysis of GLCNE in A549 cell lysate immunoprecipitated using ab189927 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.