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详细说明
概述
产品名称Anti-C1orf57抗体[EPR14325-50] - N-terminal
描述
兔单克隆抗体[EPR14325-50] to C1orf57 - N-terminal
经测试应用WB,ICC/IF,IP
种属反应性
与反应: Mouse, Rat, Human
免疫原
Recombinant fragment within Human C1orf57 aa 1-150. The exact sequence is proprietary.
Database link: Q9BSD7Run BLAST with
Run BLAST with 
阳性对照
U87-MG, Jurkat, A549 cell lysates. U87-MG and A549 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR14325-50
同种型IgG
研究领域
Cell Biology
Other Antibodies
Other Antibodies
Anti-C1orf57 antibody [EPR14325-50] - N-terminal 图像
![Western blot - Anti-C1orf57 [EPR14325-50] antibody - N-terminal (ab182155)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1821555WB.jpg)
Western blot - Anti-C1orf57 [EPR14325-50] antibody - N-terminal (ab182155)
All lanes : Anti-C1orf57 antibody [EPR14325-50] - N-terminal (ab182155) at 1/20000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 21 kDa
Observed band size : 21 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-C1orf57 [EPR14325-50] antibody - N-terminal (ab182155)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab182155IF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-C1orf57 [EPR14325-50] antibody - N-terminal (ab182155)
Immunofluorescence analysis of A549 cells, staining C1orf57 (green) with ab182155 at 1/100 dilution. An Alexa Fluor®488-conjugated goat anti rabbit IgG was used as a secondary antibody. Nuclei were counterstained with DAPI (blue).
![Immunocytochemistry/ Immunofluorescence - Anti-C1orf57 [EPR14325-50] antibody - N-terminal (ab182155)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab182155IF2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-C1orf57 [EPR14325-50] antibody - N-terminal (ab182155)
Immunofluorescence analysis of U87-MG cells, staining C1orf57 (red) with ab182155 at 1/100 dilution. An Alexa Fluor®555-conjugated goat anti rabbit IgG was used as a secondary antibody. Nuclei were counterstained with DAPI (blue).
![Immunoprecipitation - Anti-C1orf57 [EPR14325-50] antibody - N-terminal (ab182155)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1821555IP.jpg)
Immunoprecipitation - Anti-C1orf57 [EPR14325-50] antibody - N-terminal (ab182155)
Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate (lane 1) or negative control (lane 2), labeling C1orf57 immunoprecipitated using ab182155 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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