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详细说明
概述
产品名称Anti-MAGEA9 [EPR16258]抗体
描述
兔单克隆抗体[EPR16258] to MAGEA9
经测试应用Flow Cyt,IP,WB
种属反应性
与反应: Human
免疫原
Recombinant fragment within Human MAGEA9 aa 1-150. The exact sequence is proprietary.
Database link: P43362Run BLAST with
Run BLAST with 
阳性对照
K562 cell lysate: K562 cells; Human testis and melanoma lysates
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR16258
同种型IgG
研究领域
Cancer
Tumor immunology
Tumor-associated antigens
Anti-MAGEA9 [EPR16258] antibody 图像
![Immunoprecipitation - Anti-MAGEA9 [EPR16258] antibody (ab191568)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191568IP1.JPG)
Immunoprecipitation - Anti-MAGEA9 [EPR16258] antibody (ab191568)
Immunoprecipitation analysis of human testis tissue lysate labeling MAGEA9 using ab191568 at 1/100 dilution (Left Lane). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Right Lane: PBS instead of human testis tissue lysate.
![Western blot - Anti-MAGEA9 [EPR16258] antibody (ab191568)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191568WB1.JPG)
Western blot - Anti-MAGEA9 [EPR16258] antibody (ab191568)
Anti-MAGEA9 [EPR16258] antibody (ab191568) at 1/1000 dilution + K562 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 35 kDa
![Flow Cytometry - Anti-MAGEA9 [EPR16258] antibody (ab191568)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191568FC1.JPG)
Flow Cytometry - Anti-MAGEA9 [EPR16258] antibody (ab191568)
Flow cytometric analysis of 562 cells labeling MAGEA9 using ab191568 at 1/350 dilution (red) or rabbit monoclonal IgG as isotype control (green). Secondary antibody Goat anti rabbit IgG (FITC). Cells were fixed in 2% paraformaldehyde.
![Western blot - Anti-MAGEA9 [EPR16258] antibody (ab191568)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191568WB2.JPG)
Western blot - Anti-MAGEA9 [EPR16258] antibody (ab191568)
All lanes : Anti-MAGEA9 [EPR16258] antibody (ab191568) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : Human melanoma lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 35 kDa
5% NFDM/TBST blocking buffer
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