概述

• 产品名称Anti-IL23抗体[EPR5585(N)] - C-terminal
     

• 描述

  兔单克隆抗体[EPR5585(N)] to IL23 - C-terminal

• 经测试应用WB

• 种属反应性

  与反应: Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human IL23 aa 100 to the C-terminus. The exact sequence is proprietary.
  Database link: Q9NPF7

• 阳性对照    

• THP-1 treated with GM-CSF+IL-4, THP-1 and Raji cell lysates; IL23 recombinant protein (Human IL23 p40 (aa23 -aa328) & Human IL23 P19 ( aa21-aa189) (65KDa)).

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR5585(N)

• 同种型IgG

• 研究领域

• Cancer

• Tumor immunology

• Cytokines

• Interleukins

• Immunology

• Innate Immunity

• Cytokines

• Interleukins

Anti-IL23 antibody [EPR5585(N)] - C-terminal 图像

• 

  Western blot - Anti-IL23 [EPR5585(N)] antibody (ab190356)          

     All lanes : Anti-IL23 antibody [EPR5585(N)] - C-terminal (ab190356) at 1/1000 dilution
  
  Lane 1 : THP-1 treated with GM-CSF+IL-4 cell lysate
  Lane 2 : Untreated THP-1 cell lysate
  Lane 3 : Raji cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  
  Predicted band size : 21 kDa
  Observed band size : 21 kDa
   

• 

  Western blot - Anti-IL23 [EPR5585(N)] antibody (ab190356)          

     Anti-IL23 antibody [EPR5585(N)] - C-terminal (ab190356) at 1/200 dilution + IL23 recombinant protein at 0.01 µg
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  
  Predicted band size : 21 kDa
  Observed band size : 65 kDa (why is the actual band size different from the predicted?)