详细说明
概述
产品名称Anti-LHFPL5抗体[EPR16285] - C-terminal
描述
兔单克隆抗体[EPR16285] to LHFPL5 - C-terminal
经测试应用Flow Cyt,IP,WB
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LHFPL5 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: Q8TAF8阳性对照
Human fetal brain, human fetal lung, HepG2, mouse brain, mouse heart, mouse kidney, rat kidney and rat heart lysates; HepG2 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR16285
同种型IgG
Anti-LHFPL5 antibody [EPR16285] - C-terminal 图像
Immunoprecipitation - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
Western blot analysis of immunoprecipitation pellet from Human fetal brain lysate immunoprecipitated using ab192242 at 1/60 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.Flow Cytometry - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
Flow cytometric analysis of HepG2 cells (paraformaldehyde-fixed, 2%) labeling LHFPL5 with ab192242 at 1/180 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
All lanes : Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Rat heart lysate
Lane 5 : Rat kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 24 kDa
Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242) at 1/2000 dilution + HepG2 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 24 kDa
Western blot - Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242)
All lanes : Anti-LHFPL5 antibody [EPR16285] - C-terminal (ab192242) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 24 kDa