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详细说明
概述
产品名称Anti-Mimitin抗体[EP15279] - C-terminal
描述
兔单克隆抗体[EP15279] to Mimitin - C-terminal
经测试应用ICC/IF,IP,WB,IHC-P
种属反应性
与反应: Mouse, Rat, Human
免疫原
Recombinant fragment within Human Mimitin aa 50 to the C-terminus. The exact sequence is proprietary.
Database link: Q8N183Run BLAST with
Run BLAST with 
阳性对照
HepG2 cells and cell lysates: HeLa, SH-SY5Y and HEL cell lysates: Human fetal heart, Mouse kidney and Mouse heart lysates: Human liver and Mouse liver tissue.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EP15279
同种型IgG
研究领域
Metabolism
Pathways and Processes
Mitochondrial Metabolism
Oxidative phosphorylation
Complex I
Metabolism
Pathways and Processes
Mitochondrial Metabolism
Mitochondrial markers
Cancer
Tumor biomarkers
Other
Signal Transduction
Protein Trafficking
Chaperones
Other Chaperones
Signal Transduction
Metabolism
Mitochondrial
Tags & Cell Markers
Subcellular Markers
Organelles
Mitochondria
Anti-Mimitin antibody [EP15279] - C-terminal 图像
![Western blot - Anti-Mimitin antibody [EP15279] (ab192267)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192267267.JPG)
Western blot - Anti-Mimitin antibody [EP15279] (ab192267)
All lanes : Anti-Mimitin antibody [EP15279] - C-terminal (ab192267) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HEL cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 20 kDa
Observed band size : 20 kDa
![Western blot - Anti-Mimitin antibody [EP15279] (ab192267)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192267672.JPG)
Western blot - Anti-Mimitin antibody [EP15279] (ab192267)
Anti-Mimitin antibody [EP15279] - C-terminal (ab192267) at 1/10000 dilution + Human fetal heart lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 20 kDa
Observed band size : 20 kDa
![Western blot - Anti-Mimitin antibody [EP15279] (ab192267)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192267673.JPG)
Western blot - Anti-Mimitin antibody [EP15279] (ab192267)
All lanes : Anti-Mimitin antibody [EP15279] - C-terminal (ab192267) at 1/1000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 20 kDa
Observed band size : 20 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mimitin antibody [EP15279] (ab192267)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192267674.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mimitin antibody [EP15279] (ab192267)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Mimitin with ab192267 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mimitin antibody [EP15279] (ab192267)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192267675.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mimitin antibody [EP15279] (ab192267)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Mimitin with ab192267 at 1/200 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Negative control also shown.
![Immunocytochemistry/ Immunofluorescence - Anti-Mimitin antibody [EP15279] (ab192267)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192267676.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-Mimitin antibody [EP15279] (ab192267)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Mimitin with ab192267 at 1/100 dilution. The cells were permeabilised with 0.1% Triton X-100. Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/200 dilution was used as the secondary antibody (green). The nuclear counter stain is DAPI (blue).
Negative controls, ab192267 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.![Immunoprecipitation - Anti-Mimitin antibody [EP15279] (ab192267)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192267677.JPG)
Immunoprecipitation - Anti-Mimitin antibody [EP15279] (ab192267)
Western blot analysis of Mimitin in immunoprecipitation pellets from MDA-MB-435 lysate. ab192267 used at 1/60 dilution (Lane 1) or PBS (Lane 2). Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a1/1000 dilution.
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