概述

• 产品名称Anti-MLH1抗体[EPR3894]
     

• 描述

  兔单克隆抗体[EPR3894] to MLH1

• 经测试应用WB,IP,IHC-P,Flow Cyt,ICC/IF

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  A synthetic peptide corresponding to residues in Human MLH1 (UniProt ID: P40692).

• 阳性对照    

• 293T, HeLa, A431, and SW480 cell lysates; Human colonic adenocarcinoma and tonsil tissues.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

   

  Alternative versions available:
  Anti-MLH1 antibody (Alexa Fluor^® 647) [EPR3894] (ab199494)
  Anti-MLH1 antibody (Alexa Fluor^® 405) [EPR3894] (ab199495)

  Anti-MLH1 antibody (Alexa Fluor^® 488) [EPR3894] (ab199237)

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPR3894

• 同种型IgG

• 研究领域

• Cancer

• Oncoproteins/suppressors

• Tumor suppressors

• Other

• Epigenetics and Nuclear Signaling

• DNA / RNA

• DNA Damage & Repair

• Mismatch Repair

Anti-MLH1 antibody [EPR3894] 图像

• 

  Western blot - Anti-MLH1 antibody [EPR3894] (ab92312)          

     
  
  Predicted band size : 84 kDa
     

  Lane 1: Wild-type HAP1 cell lysate (20 µg)
  Lane 2: MLH1 knockout HAP1 cell lysate (20 µg)
  Lane 3: HCT116 cell lysate (20 µg)
  Lane 4: 293T cell lysate (20 µg)
  Lanes 1 - 4: Merged signal (red and green). Green - ab92312 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.
  ab92312 was shown to recognize MLH1 when MLH1 knockout samples were used, along with additional cross-reactive bands. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. ab92312 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

   

• 

  Western blot - MLH1 antibody [EPR3894] (ab92312)          

     All lanes : Anti-MLH1 antibody [EPR3894] (ab92312) at 1/10000 dilution
  
  Lane 1 : 293T cell lysate
  Lane 2 : HeLa cell lysate
  Lane 3 : A431 cell lysate
  Lane 4 : SW480 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  goat anti-rabbit HRP at 1/2000 dilution
  
  Predicted band size : 84 kDa
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MLH1 antibody [EPR3894] (ab92312)          

     ab92312 at 1/100 dilution staining MLH1 in Human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-MLH1 antibody [EPR3894] (ab92312)      Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada    

     ab92312 (1/200) staining MLH1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to abreview.    

  See Abreview

• 

  Flow Cytometry-Anti-MLH1 antibody [EPR3894](ab92312)          

     Overlay histogram showing HeLa cells stained with ab92312 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92312, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLH1 antibody [EPR3894] (ab92312)      Image from Wang X et al., PLoS One. 2011;6(10):e25913. Epub 2011 Oct 12. Fig 3.; doi:10.1371/journal.pone.0025913; October 12, 2011, PLoS ONE 6(10): e25913.    

     ab92312 staining MLH1 in Human colorectal (top) and gastric tissue (bottom) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).  

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MLH1 antibody [EPR3894] (ab92312)          

     ab92312 at 1/100 dilution staining MLH1 in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.