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详细说明
概述
产品名称Anti-heavy chain cardiac Myosin抗体[EPR10891(2)]
描述
兔单克隆抗体[EPR10891(2)] to heavy chain cardiac Myosin
经测试应用WB,IHC-P
种属反应性
与反应: Mouse, Rat, Human
免疫原
Recombinant fragment within Human heavy chain cardiac Myosin aa 1150-1350. The exact sequence is proprietary.
Database link: P12883Run BLAST with
Run BLAST with 
阳性对照
Human skeletal muscle tissue, Human, Mouse and Rat heart lysates.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR10891(2)
同种型IgG
研究领域
Cardiovascular
Heart
Contractility
Contractile Proteins
Myosins
Immunology
Immune System Diseases
Autoimmune
Signal Transduction
Cytoskeleton / ECM
Cytoskeleton
Motor Proteins
Myosin
Anti-heavy chain cardiac Myosin antibody [EPR10891(2)] 图像
![Western blot - Anti-heavy chain cardiac Myosin antibody [EPR10891(2)] (ab185967)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185967751.jpg)
Western blot - Anti-heavy chain cardiac Myosin antibody [EPR10891(2)] (ab185967)
All lanes : Anti-heavy chain cardiac Myosin antibody [EPR10891(2)] (ab185967) at 1/500 dilution
Lane 1 : Human adult heart lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat anti Rabbit IR680 at 1/10000 dilution
Predicted band size : 224 kDa
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab185967 overnight at 4°C. Antibody binding was detected using Goat anti Rabbit IR680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain cardiac Myosin [EPR10891(2)] antibody (ab185967)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185967967.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-heavy chain cardiac Myosin [EPR10891(2)] antibody (ab185967)
Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling heavy chain cardiac Myosin with ab185967 at 1/2000 dilution. Counterstained with Hematoxylin.
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