详细说明
概述
- 产品名称Anti-Ikaros抗体[EPR13791] - C-terminal
- 描述 兔单克隆抗体[EPR13791] to Ikaros - C-terminal
- 特异性Based on the sequence analysis, ab190691 recognizes seven isoforms with the predicted MWs of 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.
- 经测试应用ICC/IF, WB
- 种属反应性 与反应: Human
- 免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Ikaros aa 500 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q13422 - 阳性对照
- Jurkat , Raji and Ramos cell line lysate. Jurkat cells.
- 常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA - 纯度Protein A purified
- 克隆单克隆
- 克隆编号EPR13791
- 同种型IgG
- 研究领域
- Epigenetics and Nuclear Signaling
- Transcription
- Domain Families
- Zinc Finger
- Cancer
- Invasion/microenvironment
- Apoptosis
- Other
- Cancer
- Cell Death
- Apoptosis
- Other
Anti-Ikaros antibody [EPR13791] - C-terminal 图像
-
![Immunocytochemistry/ Immunofluorescence - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab190691691.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691)Immunofluorescent analysis of 4% paraformaldehyde fixed Jurkat cells labeling Ikaros with ab190691 at 1/250 followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and conterstained with DAPI.
-
![Western blot - Anti-Ikaros [EPR13791] antibody - C-terminal (ab190691)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1906911WB.jpg)
Western blot - Anti-Ikaros [EPR13791] antibody - C-terminal (ab190691)All lanes : Anti-Ikaros antibody [EPR13791] - C-terminal (ab190691) at 1/10000 dilution
Lane 1 : Raji cell line lysate
Lane 2 : Jurkat cell line lysate
Lane 3 : Ramos cell line lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size : 58 kDa
Observed band size : 50-70 kDa (why is the actual band size different from the predicted?)
400-6226-992