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详细说明
概述
产品名称Anti-NNF1R抗体[EPR17298]
描述
兔单克隆抗体[EPR17298] to NNF1R
经测试应用IP,WB
种属反应性
与反应: Mouse, Rat, Human
免疫原
Recombinant full length protein within Human NNF1R aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q6P1K2Run BLAST with
Run BLAST with 
阳性对照
HepG2 whole cell lysate Jurkat whole cell lysate HEK293 whole cell lysate, HeLa whole cell lysate, Human fetal brain tissue lysate, Human fetal kidney tissue lysate, C6 whole cell lysate, Raw264.7 whole cell lysate, NIH/3T3 whole cell lysate,
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR17298
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
Cell cycle
Chromosome Structure
Centromere
Cell Biology
Cell Cycle
Cell Division
Spindle
Anti-NNF1R antibody [EPR17298] 图像
![Western blot - Anti-NNF1R antibody [EPR17298] (ab199531)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199531WB1.jpg)
Western blot - Anti-NNF1R antibody [EPR17298] (ab199531)
All lanes : Anti-NNF1R antibody [EPR17298] (ab199531) at 1/1000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3 : HEK293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
developed using the ECL technique
Predicted band size : 23 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 secondsBlocking and diluting buffer was 5% NFDM /TBST.
![Western blot - Anti-NNF1R antibody [EPR17298] (ab199531)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199531WB2.jpg)
Western blot - Anti-NNF1R antibody [EPR17298] (ab199531)
All lanes : Anti-NNF1R antibody [EPR17298] (ab199531) at 1/5000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
developed using the ECL technique
Predicted band size : 23 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking and diluting buffer was 5% NFDM /TBST.
![Western blot - Anti-NNF1R antibody [EPR17298] (ab199531)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199531WB3.jpg)
Western blot - Anti-NNF1R antibody [EPR17298] (ab199531)
All lanes : Anti-NNF1R antibody [EPR17298] (ab199531) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
developed using the ECL technique
Predicted band size : 23 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 secondsBlocking and diluting buffer was 5% NFDM /TBST.
![Immunoprecipitation - Anti-NNF1R antibody [EPR17298] (ab199531)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1995311IP.jpg)
Immunoprecipitation - Anti-NNF1R antibody [EPR17298] (ab199531)
NNF1R was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab179466 at 1/80 (or 5 μg). Western blot was performed using ab199531 at 1/1000. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HeLa whole cell extract at 10ug (input)
Lane 2: HeLa whole cell extract NNF1R immunoprecipitate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199531 in HeLa whole cell extract immunoprecipitation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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