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详细说明
概述
产品名称Anti-KLC3抗体[EPR11149(2)]
描述
兔单克隆抗体[EPR11149(2)] to KLC3
经测试应用ICC/IF,WB,IHC-P,Flow Cyt,IP
种属反应性
与反应: Mouse, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human KLC3 aa 350-450. The exact sequence is proprietary.
Database link: Q6P597阳性对照
Jurkat, U87-MG, HepG2 and BxPC-3 cell lysates; Human liver tissue; HepG2 and Jurkat cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR11149(2)
同种型IgG
研究领域
Signal Transduction
Cytoskeleton / ECM
Cytoskeleton
Motor Proteins
Kinesin
Anti-KLC3 antibody [EPR11149(2)] 图像
![Western blot - Anti-KLC3 [EPR11149(2)] antibody (ab180523)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180523523.JPG)
Western blot - Anti-KLC3 [EPR11149(2)] antibody (ab180523)
All lanes : Anti-KLC3 antibody [EPR11149(2)] (ab180523) at 1/50000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : U87-MG cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : BxPC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 55 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 [EPR11149(2)] antibody (ab180523)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180523232.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 [EPR11149(2)] antibody (ab180523)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling KLC3 with ab180523 at 1/250 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG secondary and counter stained with Hematoxylin.
![Immunocytochemistry/ Immunofluorescence - Anti-KLC3 [EPR11149(2)] antibody (ab180523)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180523233.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-KLC3 [EPR11149(2)] antibody (ab180523)
Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling KLC3 with ab180523 at 1/1000 dilution followed by Goat anti-rabbit IgG (Dylight 555) at 1/250 dilution and counterstained with DAPI.
![Immunoprecipitation - Anti-KLC3 [EPR11149(2)] antibody (ab180523)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180523235.JPG)
Immunoprecipitation - Anti-KLC3 [EPR11149(2)] antibody (ab180523)
Western blot analysis of immunoprecipitation pellet from BxPC-3 cell lysate immunoprecipitated using ab180523 at 1/40 dilution (lane 1). Negative control (lane 2). Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1500 dilution was used as secondary.
![Flow Cytometry - Anti-KLC3 [EPR11149(2)] antibody (ab180523)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180523234.JPG)
Flow Cytometry - Anti-KLC3 [EPR11149(2)] antibody (ab180523)
Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling KLC3 with ab180523 at 1/10 dilution. Goat anti-rabbit IgG (FITC) at 1/75 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.
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