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详细说明
概述
产品名称Anti-KLHL6抗体[EPR13484]
描述
兔单克隆抗体[EPR13484] to KLHL6
经测试应用WB,ICC/IF,IP
种属反应性
与反应: Human
免疫原
Recombinant fragment within Human KLHL6 aa 450-600. The exact sequence is proprietary.
Database link: Q8WZ60Run BLAST with
Run BLAST with 
阳性对照
Human fetal kidney and A549 lysates; A549 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR13484
同种型IgG
研究领域
Immunology
Immunoglobulins
Receptors
Immunology
Adaptive Immunity
B Cells
Non-CD
Anti-KLHL6 antibody [EPR13484] 图像
![Immunoprecipitation - Anti-KLHL6 [EPR13484] antibody (ab182163)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1821633IP.jpg)
Immunoprecipitation - Anti-KLHL6 [EPR13484] antibody (ab182163)
Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab182163 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-KLHL6 [EPR13484] antibody (ab182163)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1821633IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KLHL6 [EPR13484] antibody (ab182163)
Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling KLHL6 with ab182163 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution. Counter stained with DAPI.
![Western blot - Anti-KLHL6 [EPR13484] antibody (ab182163)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab182163WBb.jpg)
Western blot - Anti-KLHL6 [EPR13484] antibody (ab182163)
Anti-KLHL6 antibody [EPR13484] (ab182163) at 1/1000 dilution + A549 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 70 kDa
Observed band size : 70 kDa
![Western blot - Anti-KLHL6 [EPR13484] antibody (ab182163)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1821633WB.jpg)
Western blot - Anti-KLHL6 [EPR13484] antibody (ab182163)
Anti-KLHL6 antibody [EPR13484] (ab182163) at 1/1000 dilution + Human fetal kidney lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size : 70 kDa
Observed band size : 70 kDa
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