详细说明
概述
产品名称Anti-KMT6 / EZH2抗体[EPR9307(2)] - N-terminal
描述
兔单克隆抗体[EPR9307(2)] to KMT6 / EZH2 - N-terminal
经测试应用ICC/IF,IHC-P
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human KMT6/ EZH2 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q15910阳性对照
Human breast carcinoma tissue; HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS纯度Protein A purified
克隆单克隆
克隆编号EPR9307(2)
同种型IgG
研究领域
Cancer
Tumor biomarkers
Enzymes
Other
Epigenetics and Nuclear Signaling
Chromatin Modifying Enzymes
Methylation
Lysine methylation
Epigenetics and Nuclear Signaling
Chromatin Remodeling
Polycomb Silencing
PRC3
Epigenetics and Nuclear Signaling
Chromatin Remodeling
Polycomb Silencing
PRC2
Epigenetics and Nuclear Signaling
Chromatin Modifying Enzymes
Methylation
Anti-KMT6 / EZH2 antibody [EPR9307(2)] - N-terminal 图像
Western blot - Anti-KMT6 / EZH2 [EPR9307(2)] antibody - N-terminal (ab191080)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: KMT6 / EZH2 knockout HAP1 cell lysate (20 µg)
Lane 3: HEK293 cell lysate (20 µg)
Lane 4: Ms testis tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab191080 observed at 93 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab191080 was shown to recognize KMT6 / EZH2 when KMT6 / EZH2 knockout samples were used, along with additional cross-reactive bands. Wild-type and KMT6 / EZH2 knockout samples were subjected to SDS-PAGE. ab191080 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.Immunocytochemistry/ Immunofluorescence - Anti-KMT6 / EZH2 [EPR9307(2)] antibody - N-terminal (ab191080)
Immunofluorescent analysis of HeLa cells (4% Paraformaldehyde-fixed; 0.1% tritonX-100-permeabilized) labeling KMT6 / EZH2 with ab191080 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 [EPR9307(2)] antibody - N-terminal (ab191080)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling KMT6 / EZH2 with ab191080 at 1/250 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin (inset: negative control).