![Anti-KLHL6 antibody [EPR13484] 100µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/j0an4h5gb4n.jpg)
![Anti-KLF10 antibody [EPR12102(2)] 40µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/q23tmnqzktx.JPG)
![Anti-KLF10 antibody [EPR12102(2)] 100µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/svnwlcqwhhe.JPG)
![Anti-KLC3 antibody [EPR11149(2)] 10µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/t3knb2szn20.JPG)
![Anti-KLC3 antibody [EPR11149(2)] 40µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/cotfplbrh1d.JPG)
![Anti-KLC3 antibody [EPR11149(2)] 100µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/uw1w2bi2kbu.JPG)
详细说明
概述
产品名称Anti-HMGA1抗体[EPR16649]
描述
兔单克隆抗体[EPR16649] to HMGA1
经测试应用Flow Cyt,WB,ICC/IF
种属反应性
与反应: Human
免疫原
Recombinant full length protein within Human HMGA1 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P17096Run BLAST with
Run BLAST with 
阳性对照
WB: NCCIT, BxPC-3, HepG2, SK-OV-3 and Caco-2 whole cell lysates. ICC/IF: BxPC-3 cells. Flow Cyt: HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR16649
同种型IgG
研究领域
Cancer
Tumor biomarkers
Other
Epigenetics and Nuclear Signaling
Chromatin Binding Proteins
DNA / RNA binding
Epigenetics and Nuclear Signaling
Transcription
Transcription Factors
Epigenetics and Nuclear Signaling
DNA / RNA
DNA Damage & Repair
Base Excision Repair
Microbiology
Interspecies Interaction
Host Virus Interaction
Anti-HMGA1 antibody [EPR16649] 图像
![Immunocytochemistry/ Immunofluorescence - Anti-HMGA1 antibody [EPR16649] (ab202070)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab202070070.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HMGA1 antibody [EPR16649] (ab202070)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling HMGA1 with ab202070 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing nuclear staining on BxPC-3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab202070 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.![Western blot - Anti-HMGA1 antibody [EPR16649] (ab202070)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab202070-wb.jpg)
Western blot - Anti-HMGA1 antibody [EPR16649] (ab202070)
All lanes : Anti-HMGA1 antibody [EPR16649] (ab202070) at 1/10000 dilution
Lane 1 : NCCIT (Human pluripotent embryonal carcinoma) whole cell lysate
Lane 2 : BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate
Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 4 : SK-OV-3 (Human ovarian cancer cell line) whole cell lysate
Lane 5 : Caco-2 (Human colorectal adenocarcinoma cells) whole cell lysate
Lane 6 : Human testis lysate
Lane 7 : Human lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 20 kDa
Observed band size : 18 kDa (why is the actual band size different from the predicted?)
Blocking/Dilution buffer: 5% NFDM/TBST.
![Flow Cytometry - Anti-HMGA1 antibody [EPR16649] (ab202070)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab202070-fc.jpg)
Flow Cytometry - Anti-HMGA1 antibody [EPR16649] (ab202070)
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HMGA1 with ab202070 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
粤公网安备44196802000105号
400-6226-992