概述

• 产品名称Anti-HMGA1抗体[EPR7839]
     

• 描述

  兔单克隆抗体[EPR7839] to HMGA1

• 经测试应用WB,IHC-P,Flow Cyt,ICC/IF

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human HMGA1 (N terminal).

• 阳性对照    

• WB: SK-OV-3, Caco-2, BxPC-3 and HepG2 cell lysates, rat kidney and mouse brain tissue lysates. IHC-P: Human testis and transitional cell carcinoma of bladder tissues. ICC/IF: HeLa and BxPC-3 cells. Flow Cyt: HepG2 cells.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

   

  Alternative versions available:

  Anti-HMGA1 antibody (Alexa Fluor^® 647) [EPR7839] (ab204668)

  Anti-HMGA1 antibody (Alexa Fluor^® 488) [EPR7839] (ab204667)

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR7839

• 同种型IgG

• 研究领域

• Cancer

• Tumor biomarkers

• Other

• Epigenetics and Nuclear Signaling

• Chromatin Binding Proteins

• DNA / RNA binding

• Epigenetics and Nuclear Signaling

• Transcription

• Transcription Factors

• Epigenetics and Nuclear Signaling

• DNA / RNA

• DNA Damage & Repair

• Base Excision Repair

• Microbiology

• Interspecies Interaction

• Host Virus Interaction

Anti-HMGA1 antibody [EPR7839] 图像

• 

  Western blot - Anti-HMGA1 antibody [EPR7839] (ab129153)          

     All lanes : Anti-HMGA1 antibody [EPR7839] (ab129153) at 10000 (purified)
  
  Lane 1 : HepG2 cell lysate
  Lane 2 : SK-OV-3 cell lysate
  Lane 3 : Caco-2 cell lysate
  Lane 4 : BxPC-3 cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
  
  Predicted band size : 12 kDa
  Observed band size : 17 kDa (why is the actual band size different from the predicted?)
     

  Blocking buffer and concentration: 5% NFDM/TBST.

  Diluting buffer and concentration: 5% NFDM /TBST.

   

• 

  Western blot - Anti-HMGA1 antibody [EPR7839] (ab129153)          

     Anti-HMGA1 antibody [EPR7839] (ab129153) at 1/10000 dilution (purified) + Mouse brain tissue lysate at 10 µg
  
  Secondary
  Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
  
  Predicted band size : 12 kDa
  Observed band size : 17 kDa (why is the actual band size different from the predicted?)
     

  Blocking buffer and concentration: 5% NFDM/TBST.

  Diluting buffer and concentration: 5% NFDM /TBST.

   

• 

  Western blot - Anti-HMGA1 antibody [EPR7839] (ab129153)          

     Anti-HMGA1 antibody [EPR7839] (ab129153) at 1/50000 dilution (purified) + Rat kidney tissue lysate at 10 µg
  
  Secondary
  Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
  
  Predicted band size : 12 kDa
  Observed band size : 17 kDa (why is the actual band size different from the predicted?)
     

  Blocking buffer and concentration: 5% NFDM/TBST.

  Diluting buffer and concentration: 5% NFDM /TBST.

   

• 

  Western blot - Anti-HMGA1 antibody [EPR7839] (ab129153)          

     All lanes : Anti-HMGA1 antibody [EPR7839] (ab129153) at 1/10000 dilution (unpurified)
  
  Lane 1 : SK-OV-3 cell lysate
  Lane 2 : Caco-2 cell lysate
  Lane 3 : BxPC-3 cell lysate
  Lane 4 : HepG2 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat anti-rabbit HRP at 1/2000 dilution
  
  Predicted band size : 12 kDa
  Observed band size : 17 kDa (why is the actual band size different from the predicted?)
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [EPR7839] (ab129153)          

     

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma of bladder tissue labelling HMGA1 with purified ab129153 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody [EPR7839] (ab129153)          

     

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling HMGA1 with unpurified ab129153 at 1/250.

   

• 

  Immunocytochemistry/ Immunofluorescence - Anti-HMGA1 antibody [EPR7839] (ab129153)          

     

  Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling HMGA1 with purified ab129153 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

  Control: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).

   

• 

  Immunocytochemistry/ Immunofluorescence - Anti-HMGA1 antibody [EPR7839] (ab129153)          

     

  Immunocytochemistry/Immunofluorescence analysis of BxPC-3 cells labelling HMGA1 with unpurified ab129153 at 1/250 dilution.

   

• 

  Flow Cytometry - Anti-HMGA1 antibody [EPR7839] (ab129153)          

     

  Flow cytometry analysis of HepG2 cells labelling HMGA1 with purified ab129153 at 1/60 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

   

• 

  Flow Cytometry - Anti-HMGA1 antibody [EPR7839] (ab129153)          

     

  Overlay histogram showing HepG2 cells stained with unpurified ab129153 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129153, 1/870 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.