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详细说明
概述
产品名称Anti-Natriuretic肽Receptor C抗体[EPR12716] - C-terminal
描述
兔单克隆抗体[EPR12716] to Natriuretic肽Receptor C - C-terminal
经测试应用WB,Flow Cyt
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Natriuretic Peptide Receptor C aa 500 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P17342阳性对照
Human fetal kidney, Human placenta, and HeLa lysates; HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS纯度Protein A purified
克隆单克隆
克隆编号EPR12716
同种型IgG
研究领域
Cardiovascular
Blood
Blood Pressure regulation
Signal Transduction
Cytoskeleton / ECM
Extracellular Matrix
Structures
Bone
Signal Transduction
Signaling Pathway
G Protein Signaling
GPCR
Cardiovascular
Blood
Other
Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal 图像
![Western blot - Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177954B-3.jpg)
Western blot - Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954)
All lanes : Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (purified)
Lane 1 : Human tissue lysate
Lane 2 : Human tissue lysate
Lysates/proteins at 1/10 dilution per lane.
Secondary
HRP anti-rabbit, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 60 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
![Western blot - Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177954B-2.jpg)
Western blot - Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954)
All lanes : Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954) at 1/7000 dilution (purified)
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Rat kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size : 60 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
![Western blot - Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177954B-1.jpg)
Western blot - Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954)
All lanes : Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (unpurified)
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Rat kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size : 60 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
![Flow Cytometry - Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177954-FC.jpg)
Flow Cytometry - Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954)
Overlay histogram showing HeLa cells fixed in 2% PFA and stained with purified ab177954 at a dilution of 1 in 270 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.
![Western blot - Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab177954954.jpg)
Western blot - Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954)
All lanes : Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954) at 1/1000 dilution (unpurified)
Lane 1 : Human fetal kidney lysate
Lane 2 : Human placenta lysate
Lane 3 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 60 kDa
![Flow Cytometry - Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab17/ab17795454b.jpg)
Flow Cytometry - Anti-Natriuretic Peptide Receptor C antibody [EPR12716] - C-terminal (ab177954)
Flow Cytometric analysis of permeabilized HeLa cells labeling Natriuretic Peptide Receptor C with unpurified ab177954 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
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