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详细说明
概述
产品名称Anti-JNK1 + JNK3抗体[EPR16797-194]
描述
兔单克隆抗体[EPR16797-194] to JNK1 + JNK3
经测试应用WB,IP
种属反应性
与反应: Mouse, Rat, Cow, Dog, Human, Xenopus tropicalis
免疫原
Recombinant fragment within Human JNK1 + JNK3 aa 1-400. The exact sequence is proprietary. Also P53779 (92% homology).
Database link: P45983Run BLAST with
Run BLAST with 
阳性对照
WB: Full length human JNK1 and JNK3 recombinant proteins; K562, HeLa, Jurkat, C6, RAW 264.7, PC-12, NIH/3T3, Neuro-2a, MDCK and MDBK whole cell lysates; Mouse brain, mouse heart, mouse kidney and rat kidney lysates; Xenopus (X. tropicalis) muscle lysates. IP: K562 whole cell extract.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR16797-194
同种型IgG
Anti-JNK1 + JNK3 antibody [EPR16797-194] 图像
![Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191603WBa.jpg)
Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)
All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution
Lane 1 : Full length human JNK1 recombinant protein
Lane 2 : Full length human JNK2 recombinant protein
Lane 3 : Full length human JNK3 recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 48 kDa
Observed band size : 48,71 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesRecombinant full length JNK1 protein contains aa1-427 with His-Tag®. Recombinant full length JNK2 protein contains aa1-424 with GST-tag and JNK3 protein contains aa1-464 with GST-tag.
Blocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191603WBb.jpg)
Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)
All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 48 kDa
Observed band size : 46,54 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191603WBc.jpg)
Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)
All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Rat kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 48 kDa
Observed band size : 46,54 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191603WBd.jpg)
Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)
All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 48 kDa
Observed band size : 46,54 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191603WBe.jpg)
Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)
All lanes : Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution
Lane 1 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysate
Lane 2 : Xenopus (X. tropicalis) muscle lysates
Lane 3 : MDCK (Canine kidney cell line) whole cell lysate
Lane 4 : MDBK (Bovine kidney cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 48 kDa
Observed band size : 46,54 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
![Immunoprecipitation - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1916033IP.jpg)
Immunoprecipitation - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)
JNK1 + JNK3 were immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab191603 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab191603 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: K562 whole cell extract 10 µg (Input).
Lane 2: ab191603 IP in K562 whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191603 in K562 whole cell extract.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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