概述

• 产品名称Anti-JNK1抗体[EPR140(2)]
     

• 描述

  兔单克隆抗体[EPR140(2)] to JNK1

• 经测试应用WB

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  A synthetic peptide corresponding to residues in Human JNK1.

• 阳性对照    

• HeLa, 293T, K562, and MCF7 cell lysates.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  A trial size is available to purchase for this antibody.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPR140(2)

• 同种型IgG

• 研究领域

• Immunology

• Innate Immunity

• TLR Signaling

• Cancer

• Signal transduction

• Protein phosphorylation

• Serine/threonine kinases

• MAPK pathway

• <a javascript:void(0)>Signal Transduction

• Protein Phosphorylation

• Ser / Thr Kinases

• MAPK Pathway

Anti-JNK1 antibody [EPR140(2)] 图像

• 

  Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)          

     
  
  Predicted band size : 48 kDa
     

  Lane 1: Wild-type HAP1 cell lysate (20 µg)
  Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)

  Lane 3: HeLa cell lysate (20 µg)
  Lane 4: MCF7 cell lysate (20 µg)
  Lanes 1 - 4: Merged signal (red and green). Green - ab110724 observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.
  ab110724 was shown to specifically react with JNK1 when JNK1 knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab110724 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

   

• 

  Western blot - JNK1 antibody [EPR140(2)] (ab110724)          

     All lanes : Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/1000 dilution
  
  Lane 1 : HeLa cell lysate
  Lane 2 : 293T cell lysate
  Lane 3 : K562 cell lysate
  Lane 4 : MCF7 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 48 kDa