概述

• 产品名称Anti-Histone H3 (tri methyl K79)抗体[EPR17468(2)]
     

• 描述

  兔单克隆抗体[EPR17468(2)] to Histone H3 (tri methyl K79)

• 经测试应用WB

• 种属反应性

  与反应: Mouse, Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Histone H3 aa 50 to the C-terminus (tri methyl K79). The exact sequence is proprietary.
  Database link: P68431

• 阳性对照    

• WB: HeLa and NIH/3T3 whole cell lysates; HeLa nuclear fraction.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR17468(2)

• 同种型IgG

• 研究领域

• Epigenetics and Nuclear Signaling

• Histones

• H3

• Methylated

Anti-Histone H3 (tri methyl K79) antibody [EPR17468(2)] 图像

• 

  Western blot - Anti-Histone H3 (tri methyl K79) antibody [EPR17468(2)] (ab208189)          

     All lanes : Anti-Histone H3 (tri methyl K79) antibody [EPR17468(2)] (ab208189) at 1/1000 dilution
  
  Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
  Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  developed using the ECL technique
  
  Predicted band size : 15 kDa
  Observed band size : 15 kDa
  
  
  Exposure time : 8 seconds    

  Blocking/Dilution buffer: 5% BSA/TBST.

   

• 

  Western blot - Anti-Histone H3 (tri methyl K79) antibody [EPR17468(2)] (ab208189)          

     All lanes : Anti-Histone H3 (tri methyl K79) antibody [EPR17468(2)] (ab208189) at 1/2000 dilution
  
  Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cytosolic fraction
  Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear fraction
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  developed using the ECL technique
  
  Predicted band size : 15 kDa
  Observed band size : 15 kDa
  
  
  Exposure time : 30 seconds    

  Blocking/Dilution buffer: 5% BSA/TBST.

   

• 

  Peptide Array - Anti-Histone H3 (tri methyl K79) antibody [EPR17468(2)] (ab208189)          

     

  All batches of ab208189 are tested in Peptide Array against 501 different modified and unmodified histone peptides; each peptide is printed on the array at six concentrations (each in triplicate).
   
  Circle area represents affinity between the antibody and a peptide: all antigen-containing peptides are displayed as red circles, all other peptides as blue circles. The affinity is calculated as area under curve when antibody binding values are plotted against the corresponding peptide concentration. Each circle area is normalized to the peptide with the strongest affinity.
   
  The complete dataset, including full list of all peptides and information on the position of each peptide in the diagram, can be downloaded here.