详细说明
概述
产品名称Anti-Involucrin抗体[EPR13060(N)] - C-terminal
描述
兔单克隆抗体[EPR13060(N)] to Involucrin - C-terminal
经测试应用WB,Flow Cyt
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Involucrin aa 500 to the C-terminus. The exact sequence is proprietary.
Database link: P07476阳性对照
Human fetal skin and HT-1080 cell lysates; HACAT cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR13060(N)
同种型IgG
研究领域
Cancer
Tumor biomarkers
Other
Signal Transduction
Cytoskeleton / ECM
Extracellular Matrix
Structures
Keratin
Anti-Involucrin antibody [EPR13060(N)] - C-terminal 图像
Western blot - Anti-Involucrin [EPR13060(N)] antibody - C-terminal (ab184969)
Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969) at 10000 + Human fetal skin lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 68 kDa
Flow Cytometry - Anti-Involucrin [EPR13060(N)] antibody - C-terminal (ab184969)
Flow cytometric analysis of Involucrin expression in paraformaldehyde-fixed HACAT cells using ab184969 at 1/40 dilution (red) and a rabbit IgG as negative control (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Western blot - Anti-Involucrin [EPR13060(N)] antibody - C-terminal (ab184969)
Anti-Involucrin antibody [EPR13060(N)] - C-terminal (ab184969) at 1/50000 dilution + HT-1080 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 68 kDa