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详细说明
概述
产品名称Anti-HNRNPA0抗体[EP16085]
描述
兔单克隆抗体[EP16085] to HNRNPA0
经测试应用ICC/IF,IHC-P,WB
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human HNRNPA0 aa 50-150.
Database link: Q13151阳性对照
WB: HeLa, Jurkat, A549, 293 Mouse brain, Rat brain, C6, RAW 264.7, PC-12 and NIH/3T3 lysates. IHC-P: Human cerebral cortex and Mouse spleen tissues. ICC/IF: HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EP16085
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
Chromatin Binding Proteins
DNA / RNA binding
Epigenetics and Nuclear Signaling
DNA / RNA
RNA Processing
Splicing
Anti-HNRNPA0 antibody [EP16085] 图像
![Western blot - Anti-HNRNPA0 antibody [EP16085] (ab197023)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab197023023.jpg)
Western blot - Anti-HNRNPA0 antibody [EP16085] (ab197023)
All lanes : Anti-HNRNPA0 antibody [EP16085] (ab197023) at 1/10000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) lysate
Lane 3 : A549 (Human lung carcinoma) lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 31 kDa
Observed band size : 32,34 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-HNRNPA0 antibody [EP16085] (ab197023)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab197023232.jpg)
Western blot - Anti-HNRNPA0 antibody [EP16085] (ab197023)
Anti-HNRNPA0 antibody [EP16085] (ab197023) at 1/1000 dilution + 293 (Human embryonic kidney) lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 31 kDa
Observed band size : 32,34 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-HNRNPA0 antibody [EP16085] (ab197023)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab197023233.jpg)
Western blot - Anti-HNRNPA0 antibody [EP16085] (ab197023)
All lanes : Anti-HNRNPA0 antibody [EP16085] (ab197023) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : C6 (Rat glial tumor cells) lysate
Lane 4 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate
Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) lysate
Lane 6 : NIH/3T3 (Mouse embyro fibroblast cells) lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 31 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRNPA0 antibody [EP16085] (ab197023)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab197023234.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRNPA0 antibody [EP16085] (ab197023)
Immunohistochemical analysis of paraffin-embedded Human cerebral cortex labeling HNRNPA0 with ab197023 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRNPA0 antibody [EP16085] (ab197023)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab197023235.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNRNPA0 antibody [EP16085] (ab197023)
Immunohistochemical analysis of paraffin-embedded Mouse spleen labeling HNRNPA0 with ab197023 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Mouse spleen tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-HNRNPA0 antibody [EP16085] (ab197023)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1970233IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HNRNPA0 antibody [EP16085] (ab197023)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HNRNPA0 with ab197023 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HeLa cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab197023 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
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