详细说明
概述
产品名称Anti-hnRNP Q抗体[EPR15096] - C-terminal
描述
兔单克隆抗体[EPR15096] to hnRNP Q - C-terminal
经测试应用IHC-P,ICC/IF,WB
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human hnRNP Q aa 600 to the C-terminus. The exact sequence is proprietary.
Database link: O60506阳性对照
HepG2, HCT116, HeLa and Jurkat cell lysates; Human kidney tissue; HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR15096
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
DNA / RNA
RNA Processing
Splicing
Epigenetics and Nuclear Signaling
DNA / RNA
RNA Processing
Microbiology
Interspecies Interaction
Host Virus Interaction
Anti-hnRNP Q antibody [EPR15096] - C-terminal 图像
Western blot - Anti-hnRNP Q [EPR15096] antibody (ab184946)
All lanes : Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946) at 1/50000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HTC116 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
developed using the ECL technique
Predicted band size : 70 kDa
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP Q [EPR15096] antibody - C-terminal (ab184946)
Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling hnRNP Q with ab184946 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Western blot - Anti-hnRNP Q [EPR15096] antibody (ab184946)
Anti-hnRNP Q antibody [EPR15096] - C-terminal (ab184946) at 1/10000 dilution + Jurkat cell lysate at 10 µg
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
developed using the ECL technique
Predicted band size : 70 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP Q [EPR15096] antibody (ab184946)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling hnRNP Q with ab184946 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP Q [EPR15096] antibody (ab184946)
Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling hnRNP Q with ab184946 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary at 1/200 dilution and counter-stained with DAPI (blue).