Anti-MARCKS like protein antibody [EPR15353] - C-terminal 100µl_生物试剂_其他生物试剂_生化试剂|IVD原料_生物试剂_产品

详细说明

概述

产品名称Anti-MARCKS like protein抗体[EPR15353] - C-terminal
描述
兔单克隆抗体[EPR15353] to MARCKS like protein - C-terminal
经测试应用WB,IHC-P,ICC/IF,Flow Cyt
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MARCKS like protein aa 150 to the C-terminus. The exact sequence is proprietary.
Database link: P49006
阳性对照

293T, SH-SY5Y and Human fetal brain lysates; Human colon tissue; 293T and SH-SY5Y cells.

常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
纯度Protein A purified
克隆单克隆
克隆编号EPR15353
同种型IgG
研究领域

Signal Transduction
Signaling Pathway
Calcium Signaling
Calmodulin Pathway
Signal Transduction
Protein Phosphorylation
Ser / Thr Kinases
PKC

Anti-MARCKS like protein antibody [EPR15353] - C-terminal 图像

Western blot - Anti-MARCKS like protein [EPR15353] antibody - C-terminal (ab184546)
All lanes : Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546) at 1/10000 dilution

Lane 1 : 293T cell lysate
Lane 2 : SH-SY5Y cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size : 20 kDa

The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCKS like protein [EPR15353] antibody - C-terminal (ab184546)

Immunohistochemical analysis of Paraffin-embedded Human colon tissue labeling MARCKS like protein with ab184546 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Western blot - Anti-MARCKS like protein [EPR15353] antibody - C-terminal (ab184546)
Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546) at 1/50000 dilution + Human fetal brain lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size : 20 kDa

The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation.
Immunocytochemistry/ Immunofluorescence - Anti-MARCKS like protein [EPR15353] antibody - C-terminal (ab184546)

Immunofluorescent analysis of 293T cells (4% paraformaldehyde-fixed) labeling MARCKS like protein with ab184546 at 1/250 dilution (red) followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Flow Cytometry - Anti-MARCKS like protein [EPR15353] antibody - C-terminal (ab184546)

Flow cytometric analysis of SH-SY5Y cells (2% paraformaldehyde-fixed) labeling MARCKS like protein with ab184546 at 1/140 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

• TWS用胶	• 电感胶
• 电子胶	• 环氧AB胶
• 导电银胶（晶振）	• 固晶胶
• LED 背光源固定胶	• 在线成型垫圈胶
• 导热硅脂	• 导热硅凝胶

Anti-MARCKS like protein antibody [EPR15353] - C-terminal 100µl

Anti-MARCKS like protein antibody [EPR15353] - C-terminal 100µl
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Anti-MARCKS like protein antibody [EPR15353] - C-terminal 100µl

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产品介绍

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概述

性能

Anti-MARCKS like protein antibody [EPR15353] - C-terminal 图像

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产品地区

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Anti-MARCKS like protein antibody [EPR15353] - C-terminal 100µl
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