详细说明
概述
- 产品名称Anti-KCTD5抗体[EPR16312] - C-terminal
- 描述 兔单克隆抗体[EPR16312] to KCTD5 - C-terminal
- 经测试应用Flow Cyt, ICC/IF, IHC-P, WB
- 种属反应性 与反应: Mouse, Rat, Human
- 阳性对照
- HeLa, Jurkat, RAW 264.7, C6 and NIH 3T3 whole cell lysates; Human kidney and Rat liver tissues; A431 cells; Jurkat cells.
- 常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA - 纯度Protein A purified
- 克隆单克隆
- 克隆编号EPR16312
- 同种型IgG
- 研究领域
- cript:void(0)>Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
Anti-KCTD5 antibody [EPR16312] - C-terminal 图像
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![Western blot - Anti-KCTD5 antibody [EPR16312] (ab194825)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab194825b-1.jpg)
Western blot - Anti-KCTD5 antibody [EPR16312] (ab194825)All lanes : Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825) at 1/1000 dilution
Lane 1 : HeLa whole cell lysates
Lane 2 : Jurkat whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 26 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] (ab194825)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab194825c-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] (ab194825)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 was used as secondary. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab194825.
Note: Both cytoplasm and nuclear staining on human kidney tissue. -
![Western blot - Anti-KCTD5 antibody [EPR16312] (ab194825)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab194825b-2.jpg)
Western blot - Anti-KCTD5 antibody [EPR16312] (ab194825)All lanes : Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825) at 1/1000 dilution
Lane 1 : C6 whole cell lysates
Lane 2 : RAW 264.7 whole cell lysates
Lane 3 : NIH 3T3 whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 26 kDa
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] (ab194825)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab194825c-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] (ab194825)Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 was used as secondary. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab194825.
Note: Both cytoplasm and nuclear staining on rat liver tissue. -
![Immunocytochemistry/ Immunofluorescence - Anti-KCTD5 antibody [EPR16312] (ab194825)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab194825-if.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-KCTD5 antibody [EPR16312] (ab194825)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 cells labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml), followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Counterstained with Dapi (blue). Both cytoplasm and nuclear staining on A431 cell line is observed.
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![Flow Cytometry - Anti-KCTD5 antibody [EPR16312] (ab194825)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab194825-fc.jpg)
Flow Cytometry - Anti-KCTD5 antibody [EPR16312] (ab194825)Flow cytometric analysis of Jurkat cells labeling KCTD5 with ab194825 at 1/70 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody (Blue). Rabbit monoclonal IgG was used as isotype control (Black).
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