概述

• 产品名称Anti-KCTD5抗体[EPR16312] - C-terminal
     

• 描述

  兔单克隆抗体[EPR16312] to KCTD5 - C-terminal

• 经测试应用Flow Cyt,ICC/IF,IHC-P,WB

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 阳性对照    

• HeLa, Jurkat, RAW 264.7, C6 and NIH 3T3 whole cell lysates; Human kidney and Rat liver tissues; A431 cells; Jurkat cells.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR16312

• 同种型IgG

• 研究领域

• <a javascript:void(0)>Cell Biology

• Proteolysis / Ubiquitin

• Proteasome / Ubiquitin

• Ubiquitin E3 Enzymes

• RING Finger E3 Ligase

Anti-KCTD5 antibody [EPR16312] - C-terminal 图像

• 

  Western blot - Anti-KCTD5 antibody [EPR16312] (ab194825)          

     All lanes : Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825) at 1/1000 dilution
  
  Lane 1 : HeLa whole cell lysates
  Lane 2 : Jurkat whole cell lysates
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  
  Predicted band size : 26 kDa
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] (ab194825)          

     

  Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml).  A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 was used as secondary. Counterstain: Hematoxylin.
  Inset image: negative control obtained using PBS instead of ab194825.
  Note: Both cytoplasm and nuclear staining on human kidney tissue.

   

• 

  Western blot - Anti-KCTD5 antibody [EPR16312] (ab194825)          

     All lanes : Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825) at 1/1000 dilution
  
  Lane 1 : C6 whole cell lysates
  Lane 2 : RAW 264.7 whole cell lysates
  Lane 3 : NIH 3T3 whole cell lysates
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
  
  Predicted band size : 26 kDa
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] (ab194825)          

     

  Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 was used as secondary. Counterstain: Hematoxylin.
  Inset image: negative control obtained using PBS instead of ab194825.
  Note: Both cytoplasm and nuclear staining on rat liver tissue.

   

• 

  Immunocytochemistry/ Immunofluorescence - Anti-KCTD5 antibody [EPR16312] (ab194825)          

     

  Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 cells labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml), followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Counterstained with Dapi (blue). Both cytoplasm and nuclear staining on A431 cell line is observed.

   

• 

  Flow Cytometry - Anti-KCTD5 antibody [EPR16312] (ab194825)          

     

  Flow cytometric analysis of Jurkat cells labeling KCTD5 with ab194825 at 1/70 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody (Blue). Rabbit monoclonal IgG was used as isotype control (Black).