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详细说明
概述
产品名称Anti-HNF-4-alpha抗体[EPR16786] - N-terminal
描述
兔单克隆抗体[EPR16786] to HNF-4-alpha - N-terminal
经测试应用WB,ICC/IF,IHC-P
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HNF-4-alpha aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P41235阳性对照
WB: Mouse and rat liver lysates. Human fetal liver and Human stomach lysates. HepG2 whole cell lysate. IHC-P: Human hepatocellular carcinoma tissue. ICC/IF: HepG2 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR16786
同种型IgG
研究领域
Metabolism
Types of disease
Heart disease
Metabolism
Types of disease
Diabetes
Metabolism
Pathways and Processes
Mitochondrial Metabolism
Cytochromes
Metabolism
Pathways and Processes
Metabolic signaling pathways
Energy transfer pathways
Energy Metabolism
Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Lipases
Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Cholesterol Metabolism
Metabolism
Pathways and Processes
Metabolic signaling pathways
Lipid and lipoprotein metabolism
Lipid metabolism
Cancer
Cancer Metabolism
Metabolic signaling pathway
Metabolism of lipids and lipoproteins
Developmental Biology
Organogenesis
Gut development
Liver development
Cardiovascular
Atherosclerosis
Diabetes associated
Epigenetics and Nuclear Signaling
Transcription
Polymerase associated factors
Pol II Transcription
Other
Signal Transduction
Metabolism
Energy Metabolism
Cardiovascular
Lipids / Lipoproteins
Lipid Metabolism
Cytochromes
Cardiovascular
Lipids / Lipoproteins
Lipid Metabolism
Cholesterol Metabolism
Cardiovascular
Blood
Coagulation
Extrinsic
Anti-HNF-4-alpha antibody [EPR16786] - N-terminal 图像
![Western blot - Anti-HNF-4-alpha antibody [EPR16786] (ab199431)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199431431.JPG)
Western blot - Anti-HNF-4-alpha antibody [EPR16786] (ab199431)
All lanes : Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab199431) at 1/10000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Rat liver lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Human stomach lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 53 kDa
Observed band size : kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesThis antibody can recognize 3 isoforms. The predicted MW are 53KDa, 52 KDa and 47KDa, respectively.
Blocking/dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-HNF-4-alpha antibody [EPR16786] (ab199431)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199431312.JPG)
Western blot - Anti-HNF-4-alpha antibody [EPR16786] (ab199431)
Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab199431) at 1/10000 dilution + HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 53 kDa
Observed band size : kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minuteThis antibody can recognize 3 isoforms. The predicted MW are 53KDa, 52 KDa and 47KDa, respectively.
Blocking/dilution buffer: 5% NFDM/TBST.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16786] (ab199431)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199431313.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16786] (ab199431)
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling HNF-4-alpha with ab199431 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human hepatocellular carcinoma tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).![Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16786] (ab199431)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199431314.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16786] (ab199431)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling HNF-4-alpha with ab199431 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HepG2 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1 - ab199431 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
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