详细说明
概述
- 产品名称Anti-HNF-4-alpha抗体[EPR16786] - N-terminal
- 描述 兔单克隆抗体[EPR16786] to HNF-4-alpha - N-terminal
- 经测试应用WB, ICC/IF, IHC-P
- 种属反应性 与反应: Mouse, Rat, Human
- 免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HNF-4-alpha aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P41235 - 阳性对照
- WB: Mouse and rat liver lysates. Human fetal liver and Human stomach lysates. HepG2 whole cell lysate. IHC-P: Human hepatocellular carcinoma tissue. ICC/IF: HepG2 cells.
- 常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA - 纯度Protein A purified
- 克隆单克隆
- 克隆编号EPR16786
- 同种型IgG
- 研究领域
- Cardiovascular
- Blood
- Coagulation
- Extrinsic
- Cardiovascular
- Lipids / Lipoproteins
- Lipid Metabolism
- Cholesterol Metabolism
- Cardiovascular
- Lipids / Lipoproteins
- Lipid Metabolism
- Cytochromes
- Signal Transduction
- Metabolism
- Energy Metabolism
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- Other
- Cardiovascular
- Atherosclerosis
- Diabetes associated
- Developmental Biology
- Organogenesis
- Gut development
- Liver development
- Cancer
- Cancer Metabolism
- Metabolic signaling pathway
- Metabolism of lipids and lipoproteins
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
- Metabolism
- Pathways and Processes
- Mitochondrial Metabolism
- Cytochromes
- Metabolism
- Types of disease
- Diabetes
- Metabolism
- Types of disease
- Heart disease
Anti-HNF-4-alpha antibody [EPR16786] - N-terminal 图像
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![Western blot - Anti-HNF-4-alpha antibody [EPR16786] (ab199431)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199431431.JPG)
Western blot - Anti-HNF-4-alpha antibody [EPR16786] (ab199431)All lanes : Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab199431) at 1/10000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Rat liver lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Human stomach lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 53 kDa
Observed band size : kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesThis antibody can recognize 3 isoforms. The predicted MW are 53KDa, 52 KDa and 47KDa, respectively.
Blocking/dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-HNF-4-alpha antibody [EPR16786] (ab199431)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199431312.JPG)
Western blot - Anti-HNF-4-alpha antibody [EPR16786] (ab199431)Anti-HNF-4-alpha antibody [EPR16786] - N-terminal (ab199431) at 1/10000 dilution + HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 53 kDa
Observed band size : kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minuteThis antibody can recognize 3 isoforms. The predicted MW are 53KDa, 52 KDa and 47KDa, respectively.
Blocking/dilution buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16786] (ab199431)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199431313.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HNF-4-alpha antibody [EPR16786] (ab199431)Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling HNF-4-alpha with ab199431 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human hepatocellular carcinoma tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). -
![Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16786] (ab199431)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199431314.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-HNF-4-alpha antibody [EPR16786] (ab199431)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling HNF-4-alpha with ab199431 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on HepG2 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:-
-ve control 1 - ab199431 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
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