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详细说明
概述
产品名称Anti-Hsp20抗体[EPR14457] - N-terminal
描述
兔单克隆抗体[EPR14457] to Hsp20 - N-terminal
经测试应用WB,IHC-P,ICC/IF
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Hsp20 aa 1-100. The exact sequence is proprietary.
Database link: O14558阳性对照
Human skeletal muscle and rat heart lysates; Human transitional cell carcinoma of bladder tissue; HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR14457
同种型IgG
研究领域
Cardiovascular
Heart
Apoptosis
Cardiovascular
Atherosclerosis
Ischemia / Reperfusion
Signal Transduction
Protein Trafficking
Chaperones
Heat Shock Proteins
Anti-Hsp20 antibody [EPR14457] - N-terminal 图像
![Western blot - Anti-Hsp20 [EPR14457] antibody (ab188864)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1888644WB.jpg)
Western blot - Anti-Hsp20 [EPR14457] antibody (ab188864)
Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864) at 1/10000 dilution + Human skeletal muscle lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution
Predicted band size : 17 kDa
Observed band size : 17 kDa
![Western blot - Anti-Hsp20 [EPR14457] antibody (ab188864)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab188864WBb.jpg)
Western blot - Anti-Hsp20 [EPR14457] antibody (ab188864)
Anti-Hsp20 antibody [EPR14457] - N-terminal (ab188864) at 1/1000 dilution + Rat heart lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/500 dilution
Predicted band size : 17 kDa
Observed band size : 17 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 [EPR14457] antibody (ab188864)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab188864IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp20 [EPR14457] antibody (ab188864)
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling Hsp20 with ab188864 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
![Immunocytochemistry/ Immunofluorescence - Anti-Hsp20 [EPR14457] antibody (ab188864)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1888644IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Hsp20 [EPR14457] antibody (ab188864)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Hsp20 with ab188864 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
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