概述

• 产品名称Anti-Ku70抗体[EPR4027]
     

• 描述

  兔单克隆抗体[EPR4027] to Ku70

• 经测试应用WB,IP,IHC-P,Flow Cyt,ICC/IF

• 种属反应性

  与反应: Human    
   

• 免疫原

  A synthetic peptide corresponding to residues in Human Ku70

• 阳性对照    

• HeLa, 293T, A549 and A431 cell lysates. Human testis tissue, Human tonsil tissue.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

  Alternative versions available:

  Anti-Ku70 antibody (Alexa Fluor^® 488) [EPR4027] (ab199225)

  Anti-Ku70 antibody (Alexa Fluor^® 647) [EPR4027] (ab199458)

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPR4027

• 同种型IgG

• 研究领域

• Cancer

• Cell cycle

• Cell division

• Epigenetics and Nuclear Signaling

• DNA / RNA

• DNA Damage & Repair

• Non Homol. End Joining

Anti-Ku70 antibody [EPR4027] 图像

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ku70 antibody [EPR4027] (ab92450)          

     Paraffin embedded Human testis tissue (A) or Human tonsil tissue (B) were labelled with ab92450 at 1/100 dilution.  

• 

  Western blot - Ku70 antibody [EPR4027] (ab92450)          

     All lanes : Anti-Ku70 antibody [EPR4027] (ab92450) at 1/1000 dilution
  
  Lane 1 : HeLa cell lysate
  Lane 2 : 293T cell lysate
  Lane 3 : A549 cell lysate
  Lane 4 : A431 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 70 kDa
   

• 

  Immunocytochemistry/ Immunofluorescence - Ku70 antibody [EPR4027] (ab92450)          

     Immunofluorescence staining of HeLa cells using ab92450 at 1/100 dilution.  

• 

  Flow Cytometry-Anti-Ku70 antibody [EPR4027](ab92450)          

     Overlay histogram showing HeLa cells stained with ab92450 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92450, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.