概述

• 产品名称Anti-Ku80抗体[EPR3467]
     

• 描述

  兔单克隆抗体[EPR3467] to Ku80

• 特异性The immunogen for ab79391 has 78.6% homology with mouse and rat ku80, therefore we do not guarantee reactivity in these species.

• 经测试应用WB,IHC-P,ICC,Flow Cyt

• 种属反应性

  与反应: Human    
   

• 免疫原

  A synthetic peptide corresponding to residues in human Ku80

• 阳性对照    

• A549 (treated with FBS), HeLa, U937 and HepG2 cell lysates; human breast carcinoma tissue.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

   

  Alternative versions available:

  Anti-Ku80 antibody (Alexa Fluor^® 488) [EPR3467] (ab202659)

  Anti-Ku80 antibody (HRP) [EPR3467] (ab202359)

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EPR3467

• 同种型IgG

• 研究领域

• Epigenetics and Nuclear Signaling

• DNA / RNA

• DNA Damage & Repair

• Non Homol. End Joining

Anti-Ku80 antibody [EPR3467] 图像

• 

  Flow Cytometry - Anti-Ku80 antibody [EPR3467] (ab79391)          

     Overlay histogram showing HeLa cells stained with ab79391 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79391, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.  

• 

  Western blot - Ku80 antibody [EPR3467] (ab79391)          

     All lanes : Anti-Ku80 antibody [EPR3467] (ab79391) at 1/2000 dilution
  
  Lane 1 : A549 cell lysate, treated with FBS
  Lane 2 : HeLa cell lysate
  Lane 3 : U937 cell lysate
  Lane 4 : HepG2 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  goat anti-rabbit HRP at 1/2000 dilution
  
  Predicted band size : 83 kDa
  Observed band size : 83 kDa
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ku80 antibody [EPR3467] (ab79391)          

     ab79391 at 1/250 dilution staining Ku80 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.  

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Ku80 antibody [EPR3467] (ab79391)      Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada    

     ab79391 (1/200) staining Ku80 in Hela cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.    

  See Abreview