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详细说明
概述
产品名称Anti-PGM1抗体[EPR15240]
描述
兔单克隆抗体[EPR15240] to PGM1
经测试应用WB,Flow Cyt,IP,ICC/IF
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PGM1 aa 1-100. The exact sequence is proprietary.
Database link: P36871阳性对照
Human fetal heart, fetal liver, HepG2, 293T and Jurkat lysates; 293T cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR15240
同种型IgG
研究领域
Metabolism
Pathways and Processes
Metabolic signaling pathways
Energy transfer pathways
Energy Metabolism
Signal Transduction
Metabolism
Energy Metabolism
Anti-PGM1 antibody [EPR15240] 图像
![Immunoprecipitation - Anti-PGM1 [EPR15240] antibody (ab188869)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab18886969e.jpg)
Immunoprecipitation - Anti-PGM1 [EPR15240] antibody (ab188869)
Western blot analysis of immunoprecipitation pellet from Human fetal liver lysate immunoprecipitated using ab188869 at 1/50 dilution.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.![Flow Cytometry - Anti-PGM1 [EPR15240] antibody (ab188869)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab18886969d.jpg)
Flow Cytometry - Anti-PGM1 [EPR15240] antibody (ab188869)
Flow cytometric analysis of 293T cells (paraformaldehyde-fixed, 2%) labeling PGM1 with ab188869 at 1/360 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-PGM1 [EPR15240] antibody (ab188869)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab18886969c.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PGM1 [EPR15240] antibody (ab188869)
Immunofluorescent analysis of 293T cells (4% Paraformaldehyde-fixed) labeling PGM1 with ab188869 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).
![Western blot - Anti-PGM1 [EPR15240] antibody (ab188869)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab18886969b.jpg)
Western blot - Anti-PGM1 [EPR15240] antibody (ab188869)
All lanes : Anti-PGM1 antibody [EPR15240] (ab188869) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 61 kDa
![Western blot - Anti-PGM1 [EPR15240] antibody (ab188869)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab188869869.jpg)
Western blot - Anti-PGM1 [EPR15240] antibody (ab188869)
All lanes : Anti-PGM1 antibody [EPR15240] (ab188869) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal liver lysate
Lane 3 : 293T lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/500 dilution
Predicted band size : 61 kDa
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