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详细说明
概述
产品名称Anti-PGM1抗体[EPR15241]
描述
兔单克隆抗体[EPR15241] to PGM1
经测试应用ICC/IF,IP,WB
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PGM1 aa 550 to the C-terminus. The exact sequence is proprietary.
Database link: P36871阳性对照
HepG2, 293T, Human fetal liver and fetal heart lysates; Mouse brain, heart, kidney, spleen, NIH 3T3 and RAW 264.7 lysates; Rat spleen, kidney, C6 and PC12 lysates; HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR15241
同种型IgG
研究领域
Metabolism
Pathways and Processes
Metabolic signaling pathways
Energy transfer pathways
Energy Metabolism
Signal Transduction
Metabolism
Energy Metabolism
Anti-PGM1 antibody [EPR15241] 图像
![Immunocytochemistry/ Immunofluorescence - Anti-PGM1 antibody [EPR15241] (ab192876)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192876876.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PGM1 antibody [EPR15241] (ab192876)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling PGM1 with ab192876 at 1/500 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary antibody at 1/400 dilution. Nuclear counter stain is DAPI (blue).
![Western blot - Anti-PGM1 antibody [EPR15241] (ab192876)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1928766WB.jpg)
Western blot - Anti-PGM1 antibody [EPR15241] (ab192876)
All lanes : Anti-PGM1 antibody [EPR15241] (ab192876) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Human fetal heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 61 kDa
Observed band size : 61 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-PGM1 antibody [EPR15241] (ab192876)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192876WBb.jpg)
Western blot - Anti-PGM1 antibody [EPR15241] (ab192876)
All lanes : Anti-PGM1 antibody [EPR15241] (ab192876) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat kidney lysate
Lane 6 : Rat spleen lysate
Lane 7 : C6 cell lysate
Lane 8 : RAW 264.7 cell lysate
Lane 9 : PC12 cell lysate
Lane 10 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 61 kDa
Observed band size : 61 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
![Immunoprecipitation - Anti-PGM1 antibody [EPR15241] (ab192876)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1928766IP.jpg)
Immunoprecipitation - Anti-PGM1 antibody [EPR15241] (ab192876)
Western blot analysis of PGM1 immunoprecipitated from Human fetal liver lysate with ab192876 at 1/60 dilution. Lane 1: Human fetal liver lysate. Lane 2: PBS instead of Human fetal liver lysate.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.Blocking/Dilution buffer: 5% NFDM/TBST.
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