• Species Reactivity

  Human, Mouse

• Specificity

  Detects endogenous human TRAP220 in Western blots.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human TRAP220    
  Phe878-Phe1031    
  Accession # Q15648

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAP220

TRAP220 (Thyroid hormone receptor associated protein 220 kDa) is a widely expressed member of the Mediator complex subunit 1 family of proteins. It constitutes one of some 25 subunits that make up the Mediator complex. Here, it serves as a scaffold protein that links nuclear hormone receptors to RNA polymerase II. Human TRAP220 is 1581 amino acids (aa) in length. It contains a Mediator complex interaction region (aa 108‑390) and two nuclear hormone receptor binding boxes (aa 600‑670). Phosphorylation of Thr1032 and Thr1457 materially increases TRAP220 activity. There are at least two splice variants. One shows a 22 aa substitution for aa 756‑1581 while another shows a nine aa substitution for aa 548‑1581. Over aa 878‑1031, human TRAP220 shares 92% aa identity with mouse TRAP220.

• Long Name:

  Thyroid Hormone Receptor-associated Protein Complex Component 220 kDa

• Entrez Gene IDs:

  5469 (Human); 19014 (Mouse); 497991 (Rat)

• Alternate Names:

  Activator-recruited cofactor 205 kDa component; ARC205; CRSP1TRIP2; CRSP200p53 regulatory protein RB18A; DRIP230; DRIP230PPAR-binding protein; MBD4; MED1; mediator complex subunit 1DRIP205; mediator of RNA polymerase II transcription subunit 1; PBPPPARGBP; Peroxisome proliferator-activated receptor-binding protein; PPAR binding protein; PPARBP; PPARBPMGC71488; PPARG binding protein; PPARGBP; RB18ATR-interacting protein 2; Thyroid hormone receptor-associated protein complex 220 kDa component; thyroid hormone receptor-associated protein complex component TRAP220; thyroid receptor interacting protein 2; Thyroid receptor-interacting protein 2; TRAP220; TRAP220TRIP-2; TRIP2; vitamin D receptor-interacting protein 230 kD; Vitamin D receptor-interacting protein complex component DRIP205