• Species Reactivity

  Human

• Specificity

  Detects endogenous human Eps8 in Western blots.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human Eps8    
  Met1-Ser177    
  Accession # Q12929

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Eps8

Eps8 (EGF R pathway substrate 8) is a 97 kDa member of the Eps family of proteins. It is ubiquitously expressed and serves as a signaling molecule for multiple RTKs, including FGF R, EGF R and PDGF R. Upon activation, Eps8 promotes endocytosis through Rab5, and Rac activation via IRSp53, Abi-1 and Sos-1. Human Eps8 is 822 amino acids (aa) in length. It contains a PH-like domain (aa 62‑173), an EGF R binding region (aa 296‑362), an SH3 domain that mediates homodimerization (aa 535‑587) and a Sos-1 interaction site (aa 648‑821). There is one potential alternate start site at Met261, and a splice variant that shows a 15 aa substitution for aa 676‑822.

• Long Name:

  Epidermal Growth Factor Receptor Pathway Substrate 8

• Entrez Gene IDs:

  2059 (Human); 13860 (Mouse); 312812 (Rat)

• Alternate Names:

  epidermal growth factor receptor kinase substrate 8; epidermal growth factor receptor pathway substrate 8; Eps8