• Species Reactivity

  Human, Mouse

• Specificity

  Detects human and mouse DACT3/Dapper 3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse DACT2 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant mouse DACT3/Dapper 3    
  Pro486-Lys587    
  Accession # Q0PHV7

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DACT3/Dapper 3

Dapper 3 (also Dact 3) is a 63 kDa (predicted) member of the DACT (Dapper/Frodo) family of Dvl-binding proteins.  It is expressed in cerebral cortex and uterus where it antagonizes Wnt/ beta -catenin signaling.  When present in colorectal tumor cells, it initiates apoptosis.  Both effects are likely the result of a Dapper 3 interaction with Dvl2, thereby blocking beta -catenin entry into the nucleus.  Mouse Dapper 3 is 610 amino acids (aa) in length It contains one coiled-coil region (aa 63-87), an Arg-rich domain (aa 351-476), and a PZD-binding motif (aa 607-610).  There is one potential splice variant that shows a 45 aa substitution for aa 413-610.  In human Dact 3 the frameshift at position 119 is reported to yield a protein product with estimated moleqular weight of 41 kDa. Over aa 486-587, mouse dapper 3 shares 82% and 99% aa identity with human and rat dapper 3, respectively.      

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• Long Name:

  Dapper, Antagonist of beta-Catenin, Homolog 3

• Entrez Gene IDs:

  147906 (Human); 629378 (Mouse); 499088 (Rat)

• Alternate Names:

  Antagonist of beta-catenin Dapper homolog 3; arginine rich region 1; Arginine-rich region 1 protein; DACT3; Dapper 3; dapper homolog 3; dapper, antagonist of beta-catenin, homolog 3 (Xenopus laevis); MGC15476; RRR1