• Species Reactivity

  Human

• Specificity

  Detects human Mrc2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse Mrc2 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human Mrc2    
  Cys800-Ala1059    
  Accession # Q9UBG0

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

• Immunohistochemistry

  5-15 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mrc2

Mrc2 (C-type Mannose Receptor 2; also MMR2, Endocytic Receptor 180 and uPARAP) is a 180‑200 kDa member of the macrophage mannose receptor family of proteins. It is widely expressed, being found on fibroblasts, monocytes/macrophages, endothelial cells, keratinocytes and myoepithelial cells. It binds the carbohydrates Man, Fuc and NAcGlc, type V collagen, and pro‑uPA. Mature human Mrc2 is a 1449 amino acid (aa) type I transmembrane protein. It contains a 1384 aa extracellular region and a 44 aa cytoplasmic domain. The extracellular region shows one ricin B‑type lectin domain (aa 41‑167), one fibronectin type II domain (aa 182‑230) and eight C‑type lectin domains (aa 244‑1393). Over aa 800‑1059, human Mrc2 shares 87% aa identity with mouse Mrc2.

• Long Name:

  Mannose Receptor, C-type 2

• Entrez Gene IDs:

  9902 (Human); 17534 (Mouse); 498011 (Rat)

• Alternate Names:

  CD280 antigen; CD280; CD280C-type mannose receptor 2; CLEC13E; CLEC13EUPAR-associated protein; Endo180; ENDO180C-type lectin domain family 13 member E; Endocytic receptor 180; KIAA0709FLJ35911; Macrophage mannose receptor 2; mannose receptor, C type 2; Mrc2; uPARAP; UPARAPendocytic receptor (macrophage mannose receptor family); urokinase plasminogen activator receptor-associated protein; Urokinase receptor-associated protein; Urokinase-type plasminogen activator receptor-associated protein