详细说明
Species Reactivity
Human, Mouse
Specificity
Detects human and mouse ZNF281 in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human ZNF281
aa 681-895
Accession # Q9Y2X9Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
1 µg/mL
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Western Blot | Detection of Human and Mouse ZNF281 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and A20 mouse B cell lymphoma cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), XXX µg of cytoplasmic (Cyto), and XXX µg of nuclear extracts (Nuc). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse ZNF281 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6350) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # ). A specific band was detected for ZNF281 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Preparation and Storage
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ZNF281
Long Name:
Zinc Finger Protein 281
Entrez Gene IDs:
23528 (Human); 226442 (Mouse); 305083 (Rat)
Alternate Names:
FLJ20557; GZP1; ZBP-99; zinc finger protein 416; ZNF281; ZNP-99