详细说明
概述
- 产品名称Anti-NSD3抗体[EPR13813] - N-terminal
- 描述 兔单克隆抗体[EPR13813] to NSD3 - N-terminal
- 经测试应用WB, Flow Cyt
- 种属反应性 与反应: Mouse, Rat, Human
- 免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NSD3 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9BZ95 - 阳性对照
- Hela, A673 and MCF7 lysates; HeLa cells.
- 常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS - 纯度Protein A purified
- 克隆单克隆
- 克隆编号EPR13813
- 同种型IgG
- 研究领域
- Epigenetics and Nuclear Signaling
- Chromatin Modifying Enzymes
- Methylation
- Epigenetics and Nuclear Signaling
- Chromatin Modifying Enzymes
- Methylation
- Lysine methylation
Anti-NSD3 antibody [EPR13813] - N-terminal 图像
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![Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180500B-3.jpg)
Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/50000 dilution (purified) + Rat kidney tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size : 162 kDa
Observed band size : 162 kDa
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180500B-2.jpg)
Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)All lanes : Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/10000 dilution (purified)
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size : 162 kDa
Observed band size : 162 kDa
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180500B-1.jpg)
Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)All lanes : Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/50000 dilution (purified)
Lane 1 : HeLa cell lysate
Lane 2 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size : 162 kDa
Observed band size : 162 kDa
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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![Flow Cytometry - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180500-FC.jpg)
Flow Cytometry - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)Overlay histogram showing HeLa cells fixed in 2% PFA and stained with purified ab180500 at a dilution of 1 in 70 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of primary and secondary antibody were used as a negative control (blue line).
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![Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab180500500.jpg)
Western blot - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)All lanes : Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500) at 1/50000 dilution (unpurified)
Lane 1 : Hela lysate
Lane 2 : A673 lysate
Lane 3 : MCF7 lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 162 kDa
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![Flow Cytometry - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab18050000b.jpg)
Flow Cytometry - Anti-NSD3 antibody [EPR13813] - N-terminal (ab180500)Flow Cytometrical analysis of HeLa cells labeling NSD3 with unpurified ab180500 at 1/75 (green) compared to a negative control antibody (red).
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