概述

• 产品名称Anti-Pellino 1 + Pellino 2抗体[EPR19282]

• 描述

  兔单克隆抗体[EPR19282] to Pellino 1 + Pellino 2

• 经测试应用WB

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Pellino 1 aa 150-250. The exact sequence is proprietary.
  Database link: Q96FA3

• 阳性对照    

• WB: Human Pellino 1 and Pellino 2 full length recombinant proteins; Ramos, PC-12, Jurkat, Daudi, HeLa, C6 and NIH/3T3 whole cell lysates; RAW 264.7 treated with 1 µg/ml LPS for 6 hours whole cell lysate; Mouse and Rat brain lysates.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR19282

• 同种型IgG

• 研究领域

• Epigenetics and Nuclear Signaling

• Cell cycle

• Kinases/Phosphatases

• Other

• Signal Transduction

• Signaling Pathway

• Nuclear Signaling

• NFkB Pathway

• Signal Transduction

• Protein Phosphorylation

• Ser / Thr Kinases

• Other Kinases

• Signal Transduction

• Protein Phosphorylation

• Ser / Thr Kinases

• MAPK Pathway

Anti-Pellino 1 + Pellino 2 antibody [EPR19282] 图像

• 

  Western blot - Anti-Pellino 1 antibody [EPR19282] (ab192833)          

     All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/5000 dilution
  
  Lane 1 : Human Pellino 1 full length recombinant protein
  Lane 2 : Human Pellino 2 full length recombinant protein
  Lane 3 : Human Pellino 3 full length recombinant protein
  
  Lysates/proteins at 0.01 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  
  Predicted band size : 46 kDa
  Observed band size : 46 kDa
     

  Blocking/dilution buffer: 5% NFDM/TBST.

  Exposure times: Lane 1:1 second; Lane 2 and 3:1 minute.

  Human Pellino 1 full length recombinant protein contains aa1-418 with a His-Tag®. Human Pellino 2 full length recombinant protein contains aa1-420 with a His-Tag®. Human Pellino 3  full length recombinant protein contains aa1-469 with a His-Tag®. All three recombinant proteins were made in-house.

   

• 

  Western blot - Anti-Pellino 1 antibody [EPR19282] (ab192833)          

     All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution
  
  Lane 1 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
  Lane 2 : Untreated RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
  Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 1 µg/ml LPS for 6 hours, whole cell lysate
  Lane 4 : Untreated PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
  Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) treated with 1 µg/ml LPS for 6 hours whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  
  Predicted band size : 46 kDa
  Observed band size : 46 kDa
  
  
  Exposure time : 3 minutes    

  Blocking/dilution buffer: 5% NFDM/TBST.

  The expression of Pellino 1 can be induced by LPS. (PMID: 21204785).

   

• 

  Western blot - Anti-Pellino 1 antibody [EPR19282] (ab192833)          

     All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution
  
  Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
  Lane 2 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate
  Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  
  Predicted band size : 46 kDa
  Observed band size : 46 kDa
  
  
  Exposure time : 3 minutes    

  Blocking/dilution buffer: 5% NFDM/TBST.

   

• 

  Western blot - Anti-Pellino 1 antibody [EPR19282] (ab192833)          

     All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution
  
  Lane 1 : Mouse brain lysate
  Lane 2 : Rat brain lysate
  Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
  Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
  
  Predicted band size : 46 kDa
  Observed band size : 46 kDa
  
  
  Exposure time : 3 minutes    

  Blocking/dilution buffer: 5% NFDM/TBST.