详细说明
概述
- 产品名称Anti-PNK抗体[EPR11935] - C-terminal
- 描述 兔单克隆抗体[EPR11935] to PNK - C-terminal
- 经测试应用IHC-P, WB, IP
- 种属反应性 与反应: Mouse, Human
- 免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PNK aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: Q96T60 - 阳性对照
- HeLa, PC-3, Jurkat and Human testis lysate. Human breast and pancreas tissue. HeLa cells.
- 常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
- 存储溶液Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA - 纯度Tissue culture supernatant
- 克隆单克隆
- 克隆编号EPR11935
- 同种型IgG
- 研究领域
- Epigenetics and Nuclear Signaling
- DNA / RNA
- DNA Damage & Repair
- DNA Damage Response
- Other
Anti-PNK antibody [EPR11935] - C-terminal 图像
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![Western blot - Anti-PNK [EPR11935] antibody - C-terminal (ab181107)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181107072.jpg)
Western blot - Anti-PNK [EPR11935] antibody - C-terminal (ab181107)All lanes : Anti-PNK antibody [EPR11935] - C-terminal (ab181107) at 1/2000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Human testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 57 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK [EPR11935] antibody - C-terminal (ab181107)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181107074.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK [EPR11935] antibody - C-terminal (ab181107)Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PNK with ab181107 at 1/100 dilution. Counter stain Hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK [EPR11935] antibody - C-terminal (ab181107)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181107073.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK [EPR11935] antibody - C-terminal (ab181107)Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling PNK with ab181107 at 1/100 dilution. Counter stain Hematoxylin.
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![Western blot - Anti-PNK [EPR11935] antibody - C-terminal (ab181107)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181107107.jpg)
Western blot - Anti-PNK [EPR11935] antibody - C-terminal (ab181107)All lanes : Anti-PNK antibody [EPR11935] - C-terminal (ab181107) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : PC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 57 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
-
![Immunoprecipitation - Anti-PNK [EPR11935] antibody - C-terminal (ab181107)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181107075.jpg)
Immunoprecipitation - Anti-PNK [EPR11935] antibody - C-terminal (ab181107)Western blot analysis of PNK in immunoprecipitation pellets from HeLa cell lysate. ab181107 used at 1/20 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500. Right lane shows negative control.
400-6226-992