详细说明
概述
产品名称Anti-PNK抗体[EPR11935] - C-terminal
描述
兔单克隆抗体[EPR11935] to PNK - C-terminal
经测试应用IHC-P,WB,IP
种属反应性
与反应: Mouse, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PNK aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: Q96T60阳性对照
HeLa, PC-3, Jurkat and Human testis lysate. Human breast and pancreas tissue. HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR11935
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
DNA / RNA
DNA Damage & Repair
DNA Damage Response
Other
Anti-PNK antibody [EPR11935] - C-terminal 图像
Western blot - Anti-PNK [EPR11935] antibody - C-terminal (ab181107)
All lanes : Anti-PNK antibody [EPR11935] - C-terminal (ab181107) at 1/2000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Human testis lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 57 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK [EPR11935] antibody - C-terminal (ab181107)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PNK with ab181107 at 1/100 dilution. Counter stain Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PNK [EPR11935] antibody - C-terminal (ab181107)
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling PNK with ab181107 at 1/100 dilution. Counter stain Hematoxylin.
Western blot - Anti-PNK [EPR11935] antibody - C-terminal (ab181107)
All lanes : Anti-PNK antibody [EPR11935] - C-terminal (ab181107) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : PC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 57 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
Immunoprecipitation - Anti-PNK [EPR11935] antibody - C-terminal (ab181107)
Western blot analysis of PNK in immunoprecipitation pellets from HeLa cell lysate. ab181107 used at 1/20 dilution. Secondary antibody Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500. Right lane shows negative control.