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详细说明
概述
产品名称Anti-PP11抗体[EPR15137] - N-terminal
描述
兔单克隆抗体[EPR15137] to PP11 - N-terminal
经测试应用IP,WB,IHC-P
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PP11 aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: P21128阳性对照
Human placenta, skin and fetal skin lysates; Human placenta tissue.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR15137
同种型IgG
研究领域
Cell Biology
Proteolysis / Ubiquitin
Proteolytic enzymes
Other proteases
Epigenetics and Nuclear Signaling
DNA / RNA
RNA Processing
Other
Anti-PP11 antibody [EPR15137] - N-terminal 图像
![Western blot - Anti-PP11 [EPR15137] antibody (ab185209)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab1852099wb.JPG)
Western blot - Anti-PP11 [EPR15137] antibody (ab185209)
All lanes : Anti-PP11 antibody [EPR15137] - N-terminal (ab185209) at 1/10000 dilution
Lane 1 : Human placenta lysate
Lane 2 : Human skin lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 47 kDa
Based on the sequence analysis, ab185209 recognizes two isoforms with the predicted MWs of 42KDa and 47KDa, respectively.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP11 [EPR15137] antibody (ab185209)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185209ihc.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PP11 [EPR15137] antibody (ab185209)
Immunohistochemical analysis of Paraffin-embedded Human placenta tissue labeling PP11 with ab185209 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
![Immunoprecipitation - Anti-PP11 [EPR15137] antibody - N-terminal (ab185209)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185209opy.jpg)
Immunoprecipitation - Anti-PP11 [EPR15137] antibody - N-terminal (ab185209)
Western blot analysis of immunoprecipitation pellet from Human fetal skin lysate immunoprecipitated using ab185209 at 1/50 dilution (lane 1) or PBS control (lane 2).
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
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