详细说明
- Species ReactivityHuman
- SpecificityDetects human PS-1 NTF in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) PS-1 CTF (aa 298-407) and rhPS-2 NTF (aa 1-80) is observed.
- SourcePolyclonal Goat IgG
- PurificationAntigen Affinity-purified
- ImmunogenE. coli-derived recombinant human Presenilin-1
Lys1-Lys80
Accession # P49768 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
Applications
- Recommended
ConcentrationSample
- Western Blot0.1 µg/mLRecombinant Human Presenilin‑1 N-Terminal Fragment
- Immunohistochemistry5-15 µg/mLSee below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
| Immunohistochemistry | Presenilin‑1 in Human Brain. Presenilin‑1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using 15 µg/mL Goat Anti-Human Presenilin‑1 N‑Terminal Fragment Antigen Affinity-purified Polyclonal Antibody (Catalog # AF149) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ) and counterstained with hematoxylin (blue). View our protocol for . |
Preparation and Storage
- ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Presenilin-1
Presenilin-1 (PS-1) and Presenilin-2 (PS-2) are integral membrane proteins that contain multiple transmembrane domains. Both are predominantly located within the endoplasmic reticulum (ER) and early Golgi apparatus. They are primarily expressed in neurons and are ubiquitously expressed in the brain. PS-1 and PS-2 are proteolytically cleaved to generate an N-terminal fragment (NTF) and a C-terminal fragment (CTF). Mutations in the gene for Presenilin may play an important role in the pathology of Alzheimer's disease.
- Entrez Gene IDs:5663 (Human)
- Alternate Names:AD3EC 3.4.23.-; Alzheimer disease 3; EC 3.4.23; FAD; presenilin 1; Presenilin1; Presenilin-1; Protein S182; PS-1; PS1presenilin-1; PSEN1; PSNL1; S182
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