详细说明
- Species ReactivityHuman
- SpecificityDetects human Presenilin‑2 N-Terminal Fragment in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Presenilin‑1 N-Terminal Fragment (aa 1‑80) and recombinant human Presenilin‑2 C-Terminal Fragment (aa 308‑388) is observed.
- SourcePolyclonal Goat IgG
- PurificationAntigen Affinity-purified
- ImmunogenE. coli-derived recombinant human Presenilin-2
Leu2-Thr80
Accession # P49810 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Recommended
ConcentrationSample
- Western Blot0.1 µg/mLRecombinant Human Presenilin‑2 N-Terminal Fragment
- Immunohistochemistry5-15 µg/mLImmersion fixed paraffin-embedded sections of human brain (hippocampus)
- ReconstitutionReconstitute at 0.2 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Presenilin‑2 (PS-2 and PSEN2) is a catalytic subunit of the gamma-secretase complex. Presenilin‑2 may play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Heterogeneous proteolytic processing of Presenilin‑2 generates N-terminal (aa 1-297) and C-terminal (aa 298-248) fragments. Presenilin 1 and Presenilin 2 determine gamma-secretase activity, which is responsible for cleaving the amyloid precursor protein (APP). In turn, APP cleavage can produce amyloid-beta, which is the primary component of plaques in the brains of Alzheimer’s disease patients. Full length, approximately 50 kDa, and the approximately 30 kDa N-terminal fragment of Presenilin-2 were identified by western blotting in extracted rodent pancreas but only the 30 kDa fragment was detected in mouse islets and human insulinoma. Within a sequence correspondent to the immunogen, human PS-2 shares 87% aa sequence identity with mouse and rat PS-2.
- Entrez Gene IDs:5664 (Human)
- Alternate Names:AD3LP; AD4AD3L; AD5; E5-1; EC 3.4.23; EC 3.4.23.-; presenilin 2 (Alzheimer disease 4); Presenilin2; Presenilin-2; PS-2; PS2CMD1V; PSEN2; PSNL2; STM2STM-2
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