详细说明
概述
产品名称Anti-Pigu抗体[EPR16424] - C-terminal
描述
兔单克隆抗体[EPR16424] to Pigu - C-terminal
经测试应用IHC-P,WB
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Pigu aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: Q9H490阳性对照
HeLa, 293, Human fetal brain, C6, RAW 264.7, PC12 and NIH 3T3 lysates; Human liver and mouse kidney tissues.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR16424
同种型IgG
研究领域
Tags & Cell Markers
Subcellular Markers
Organelles
ER
Anti-Pigu antibody [EPR16424] - C-terminal 图像
![Western blot - Anti-Pigu antibody [EPR16424] - C-terminal (ab192255)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192255551.jpg)
Western blot - Anti-Pigu antibody [EPR16424] - C-terminal (ab192255)
Anti-Pigu antibody [EPR16424] - C-terminal (ab192255) at 1/10000 dilution + HeLa cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 50 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pigu antibody [EPR16424] - C-terminal (ab192255)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192255555.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pigu antibody [EPR16424] - C-terminal (ab192255)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Pigu with ab192255 at 1/1000 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
![Western blot - Anti-Pigu antibody [EPR16424] - C-terminal (ab192255)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192255552.jpg)
Western blot - Anti-Pigu antibody [EPR16424] - C-terminal (ab192255)
All lanes : Anti-Pigu antibody [EPR16424] - C-terminal (ab192255) at 1/1000 dilution
Lane 1 : HT-1376 cell lysate
Lane 2 : 293 cell lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 50 kDa
![Western blot - Anti-Pigu antibody [EPR16424] - C-terminal (ab192255)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192255553.jpg)
Western blot - Anti-Pigu antibody [EPR16424] - C-terminal (ab192255)
All lanes : Anti-Pigu antibody [EPR16424] - C-terminal (ab192255) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 50 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pigu antibody [EPR16424] - C-terminal (ab192255)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab192255554.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pigu antibody [EPR16424] - C-terminal (ab192255)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Pigu with ab192255 at 1/1000 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
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