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详细说明
概述
产品名称Anti-NR0B1 / Dax1抗体[EP13786] - N-terminal
描述
兔单克隆抗体[EP13786] to NR0B1 / Dax1 - N-terminal
经测试应用ICC/IF,IHC-P,WB
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NR0B1/ Dax1 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P51843阳性对照
A431 and Human testis lysates; Human testis tissue; SH-SY5Y cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EP13786
同种型IgG
研究领域
Developmental Biology
Reproduction
Placental development
Developmental Biology
Reproduction
Sex determination
Epigenetics and Nuclear Signaling
Nuclear Signaling Pathways
Nuclear Receptors
Orphan Nuclear Receptors
Epigenetics and Nuclear Signaling
Nuclear Signaling Pathways
Nuclear Receptors
Retinoic & Retinoid
Neuroscience
Endocrine system
Hypothalamic pituitary adrenal axis
Neuroscience
Endocrine system
Gonadotrophic axis
Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal 图像
![Immunocytochemistry/ Immunofluorescence - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19664949d.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
Immunofluorescent analysis of SH-SY5Y cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling NR0B1 / Dax1 with ab196649 at 1/300 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).
Note: Nuclear and cytoplasm staining on SH-SY5Y cell line was observed.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19664949c.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling NR0B1 / Dax1 with ab196649 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Nuclear staining on human testis tissue was observed.
![Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab19664949b.jpg)
Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649) at 1/1000 dilution + Human testis lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 52 kDa
![Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab196649649.jpg)
Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649) at 1/1000 dilution + A431 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size : 52 kDa
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