• Species Reactivity

  Human

• Specificity

  Detects human and mouse CtBP1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human CtBP2 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human CtBP1    
  Met1-Leu440    
  Accession # Q13363

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CtBP1

CtBP1 (C terminal binding protein 1) was originally identified through its binding to the Adenovirus E1A oncoprotein via a 5 amino acid motif, PLDLS, which repressed E1A induced oncogenesis and cellular transformation. In the nucleus, CtBP1 functions predominantly as a transcriptional corepressor in conjunction with a variety of DNA binding repressors.Interaction with Pnn/DRS may modulate repression of E-cadherin by CtBP1. Recruitment of CtBP1 and HDAC to Smad1/Smad4 complexes by Smad6 results in inhibition of BMP stimulated gene expression. The interaction of CtBP1 with polycomb group proteins suggests it is a component of the chromatin remodeling machinery and transcriptional repression. Cytosolic CtBP1 has also been shown to be involved with CNS synapses, membrane trafficking, and regulation of the microtubule cytoskeleton.

• Long Name:

  C-terminal-Binding Protein 1

• Entrez Gene IDs:

  1487 (Human); 13016 (Mouse); 29382 (Rat)

• Alternate Names:

  BARS; brefeldin A-ribosylated substrate; CTBP; CtBP1; C-terminal binding protein 1; C-terminal-binding protein 1; EC 1.1.1; EC 1.1.1.-; EC 1.1.1.158; MGC104684