详细说明
概述
产品名称Anti-PRC1 (phospho T481)抗体[EP1514Y]
描述
兔单克隆抗体[EP1514Y] to PRC1 (phospho T481)
特异性ab62366 detects PRC1 phosphorylated at Theonine 481.
经测试应用WB,IHC-P,ICC
种属反应性
与反应: Human
免疫原
A phosphopeptide corresponding to residues surrounding Threonine 481 of human PRC1
阳性对照
HeLa cells and HeLa cell lysates.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
形式Liquid
存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
纯度Tissue culture supernatant
克隆单克隆
克隆编号EP1514Y
同种型IgG
研究领域
<a javascript:void(0)>Cell Biology
Cell Cycle
Cell Division
Cytokinesis
Anti-PRC1 (phospho T481) antibody [EP1514Y] 图像
Western blot - PRC1 (phospho T481) antibody [EP1514Y] (ab62366)
All lanes : Anti-PRC1 (phospho T481) antibody [EP1514Y] (ab62366) at 1/500 dilution
Lane 1 : HeLa cell lysates (untreated)
Lane 2 : HeLa cell lysates treated with aphidicolin + nocodazole
Lysates/proteins at 10 µg per lane.
Secondary
goat anti-rabbit HRP labeled, at 1/2000 dilution
Predicted band size : 72 kDa
Observed band size : 72 kDa
Immunocytochemistry - PRC1 (phospho T481) antibody [EP1514Y] (ab62366)
ab62366 at 1/100 dilution staining PRC1 in HeLa cells by Immunocytochemistry.