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详细说明
概述
产品名称Anti-PAH [EPR12381]抗体- C-terminal
描述
兔单克隆抗体[EPR12381] to PAH - C-terminal
经测试应用Flow Cyt,WB,IHC-P
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PAH aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P00439阳性对照
HeLa, 293T, HepG2, RAW 264.7, C6, NIH 3T3, PC12, Human fetal liver and U87-MG whole cell lysatse; Human liver and Mouse kidney tissues; U87-MG cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR12381
同种型IgG
研究领域
Metabolism
Pathways and Processes
Metabolic signaling pathways
Amino acid metabolism
Signal Transduction
Metabolism
Amino Acids
Anti-PAH [EPR12381] antibody - C-terminal 图像
![Western blot - Anti-PAH [EPR12381] antibody - C-terminal (ab191415)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191415WB2.jpg)
Western blot - Anti-PAH [EPR12381] antibody - C-terminal (ab191415)
All lanes : Anti-PAH [EPR12381] antibody - C-terminal (ab191415) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 52 kDa
![Flow Cytometry - Anti-PAH [EPR12381] antibody - C-terminal (ab191415)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1914152FC.jpg)
Flow Cytometry - Anti-PAH [EPR12381] antibody - C-terminal (ab191415)
Flow cytometric analysis of U87-MG cells (paraformaldehyde-fixed, 2%) labeling PAH with ab191415 at 1/300 dilution (red), or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH [EPR12381] antibody - C-terminal (ab191415)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191415HC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH [EPR12381] antibody - C-terminal (ab191415)
Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling PAH with ab191415 at 1/800 dilution (1.9 μg/ml) followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Inset: Negative control: using PBS instead of primary antibody.![Western blot - Anti-PAH [EPR12381] antibody - C-terminal (ab191415)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1914152WB.jpg)
Western blot - Anti-PAH [EPR12381] antibody - C-terminal (ab191415)
All lanes : Anti-PAH [EPR12381] antibody - C-terminal (ab191415) at 1/10000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : HepG2 cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size : 52 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH [EPR12381] antibody - C-terminal (ab191415)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab191415IHC.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAH [EPR12381] antibody - C-terminal (ab191415)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling PAH with ab191415 at 1/800 dilution (1.9 μg/ml) followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Inset: Negative control: using PBS instead of primary antibody.
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